公开(公告)号：US06989162B2

公开(公告)日：2006-01-24

申请号：US10403700

申请日：2003-03-31

Applicant: Ghulam Nabi Qazi ,  Om Parkash Suri ,  Kasturi Lal Bedi ,  Krishan Avtar Suri ,  Bishan Datt Gupta ,  Bupinder Singh Jaggi ,  Bal Krishan Kapahi ,  Naresh Kumar Satti ,  Musarat Amina ,  Bal Krishan Chandan ,  Neelam Sharma ,  Gurdarshan Singh

Inventor： Ghulam Nabi Qazi ,  Om Parkash Suri ,  Kasturi Lal Bedi ,  Krishan Avtar Suri ,  Bishan Datt Gupta ,  Bupinder Singh Jaggi ,  Bal Krishan Kapahi ,  Naresh Kumar Satti ,  Musarat Amina ,  Bal Krishan Chandan ,  Neelam Sharma ,  Gurdarshan Singh

IPC: A61K35/78 ,  A61K31/70 ,  A61K31/33 ,  A61K31/555 ,  A61K31/05

CPC classification number: A61K36/53 ,  Y10S514/893

Abstract: A process of isolation of pure Acteoside of high hepatoprotection from plant Colerbrookea oppositifolia, said process comprising steps of drying aerial parts of the plant, grounding the dried parts into powder, percolating the powder with water or ethanol for 3-4 times to obtain an extract, filtering the extract for clearing of suspended particles to obtain supernatant, drying the supernatant at about 45 to 55° C. to obtain a residue, fractionating the residue with chloroform, ethyl acetate, and butanol successively, subjecting butanol fraction to adsorption chromatography of SiO2 after adding methanol to the fraction, charging the adsorbed fraction to glass column, eluting the column with solvents of increasing polarity of methanol:chloroform to obtain further fractions and repeating the process one more time, subjecting the fractions to column chromatography to obtain fractions, concentrating the fractions under reduced pressure to obtain acteoside as residue; and a method of effectively hepatoprotecting a subject using pure Acteoside from plant Colerbrookea oppositifolia, said method comprising steps of administering appropriate low-dose of the acteoside to the subject.

Abstract translation: 从植物Colerbrookea oppositifolia中分离出高保护酶的纯Acteoside的过程，所述方法包括干燥植物的地上部分，将干燥部分粉碎成粉末，用水或乙醇渗滤粉末3-4次以获得提取物 过滤提取物以清除悬浮颗粒以获得上清液，在约45至55℃下干燥上清液，得到残余物，依次用氯仿，乙酸乙酯和丁醇分馏残余物，将丁醇级分进行吸附层析 在将甲醇加入到级分中后，将吸附的级分装入玻璃柱，用极性升高的甲醇：氯仿溶剂洗脱柱，得到更多的级分并重复该过程一次， 进行柱色谱分离，得到级分，减压浓缩，得到作为残留物的acteoside; 该方法包括以下步骤：向受试者施用合适的低剂量的acteoside。