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公开(公告)号：US20110065099A1
公开(公告)日：2011-03-17
申请号：US12949446
申请日：2010-11-18
申请人： Kyouichi OHSHIRO , Kazuhiro OHMIYA , Naoko IZUI
发明人： Kyouichi OHSHIRO , Kazuhiro OHMIYA , Naoko IZUI
IPC分类号： C12Q1/70 , G01N33/566
CPC分类号： G01N33/56983 , G01N33/5306 , G01N2333/11 , G01N2333/95
摘要： The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.
摘要翻译： 本发明提供了一种预处理样品的方法，其允许根据免疫测定进行测量，以在防止非特异性反应的同时进行来自鼻分泌物的样品。 根据该方法，预先用蛋白酶处理来自鼻分泌物的样品，然后进行免疫测定。 作为蛋白酶，优选使用半碱性蛋白酶（EC 3.4.21.63）。 此外，优选通过本发明的预处理方法进行预处理的物质是来自鼻分泌物的样品中含有的流感病毒。 免疫测定优选是免疫凝集测定。 免疫凝集测定的实例包括比浊免疫测定，胶乳比浊免疫测定和在载玻片上进行的胶乳凝集测定。
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公开(公告)号：US08426125B2
公开(公告)日：2013-04-23
申请号：US12949446
申请日：2010-11-18
申请人： Kyouichi Ohshiro , Kazuhiro Ohmiya , Naoko Izui
发明人： Kyouichi Ohshiro , Kazuhiro Ohmiya , Naoko Izui
CPC分类号： G01N33/56983 , G01N33/5306 , G01N2333/11 , G01N2333/95
摘要： The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.
摘要翻译： 本发明提供了一种预处理样品的方法，其允许根据免疫测定进行测量，以在防止非特异性反应的同时进行来自鼻分泌物的样品。 根据该方法，预先用蛋白酶处理来自鼻分泌物的样品，然后进行免疫测定。 作为蛋白酶，优选使用半碱性蛋白酶（EC 3.4.21.63）。 此外，优选通过本发明的预处理方法进行预处理的物质是来自鼻分泌物的样品中含有的流感病毒。 免疫测定优选是免疫凝集测定。 免疫凝集测定的实例包括比浊免疫测定，胶乳比浊免疫测定和在载玻片上进行的胶乳凝集测定。
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公开(公告)号：US20080008992A1
公开(公告)日：2008-01-10
申请号：US11792990
申请日：2005-12-13
申请人： Kyouichi Ohshiro , Kazuhiro Ohmiya , Naoko Izui
发明人： Kyouichi Ohshiro , Kazuhiro Ohmiya , Naoko Izui
CPC分类号： G01N33/56983 , G01N33/5306 , G01N2333/11 , G01N2333/95
摘要： The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.
摘要翻译： 本发明提供了一种预处理样品的方法，其允许根据免疫测定进行测量，以在防止非特异性反应的同时进行来自鼻分泌物的样品。 根据该方法，预先用蛋白酶处理来自鼻分泌物的样品，然后进行免疫测定。 作为蛋白酶，优选使用半碱性蛋白酶（EC 3.4.21.63）。 此外，优选通过本发明的预处理方法进行预处理的物质是来自鼻分泌物的样品中含有的流感病毒。 免疫测定优选是免疫凝集测定。 免疫凝集测定的实例包括比浊免疫测定，胶乳比浊免疫测定和在载玻片上进行的胶乳凝集测定。
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