-
公开(公告)号：US06720162B2
公开(公告)日：2004-04-13
申请号：US09871424
申请日：2001-05-31
IPC分类号： C12Q142
摘要： Disclosed is a method and corresponding kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.
-
公开(公告)号：US06893834B2
公开(公告)日：2005-05-17
申请号：US10693214
申请日：2003-10-24
IPC分类号： G01N33/53 , C12Q1/42 , C12Q1/48 , G01N33/543 , G01N33/573
摘要： Disclosed is a method and corresponding kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.
摘要翻译： 公开了用于测定分类在选自EC 2.7.1，EC 3.1.3和EC 3.1.4的酶分类内的酶的存在，活性或两者的方法和相应试剂盒。 该方法通常包括使酶与底物反应足以产生磷酸化或脱磷酸化产物的步骤; 使产物与结合基质接触，由此产物粘附到基质上; 然后分析该基质是否存在固定在基质上的产物的存在和量的存在和数量，从而可以确定酶的存在，活性或存在和活性。
-
搜索结果
2 条记录 (耗时 0.009 秒)
