公开(公告)号：US20240344135A1

公开(公告)日：2024-10-17

申请号：US18294652

申请日：2022-08-05

申请人： Agency for Science, Technology and Research ,  Nation University Hospital (Singapore) Pte Ltd

发明人： Lian Chye Winston Koh ,  Peiling Yang

IPC分类号： C12Q1/6886 ,  C12Q1/6806

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： There is provided a method of detecting and/or determining the presence of one or more thyroid-specific nucleic acid, the method comprising annealing the one or more thyroid-specific nucleic acid in the presence of a control nucleic acid, and subjecting each of the one or more thyroid-specific nucleic acid to one or more amplification step in the presence of a mixture comprising a surfactant and an oligonucleotide primer and/or probe capable of hybridizing with the one or more thyroid-specific nucleic acid, wherein the oligonucleotide primer and/or probe comprises a cleavage site and a cleavable 3′ end. Also disclosed are methods of detecting and/or determining thyroid cancer recurrence and/or metastasis and thyroid-specific nucleic acid detection mixtures, and kits thereof.