公开(公告)号：US20240345087A1

公开(公告)日：2024-10-17

申请号：US18633527

申请日：2024-04-12

申请人： Juye Xinhao Agriculture and Animal Husbandry Co. LTD ,  New Hope Liuhe Co., Ltd ,  Xiajin New hope Liuhe agriculture and animal husbandry Co., LTD ,  Shandong new hope Liuhe Group Co., Ltd ,  Shandong New hope Liuhe Agriculture and Animal Husbandry Technology Co. LTD ,  New Hope Group Limited

发明人： Xiaowen Li ,  Jiapei Huang ,  Yang Li ,  Mengli QIAO ,  Jie Qing ,  Zewei WANG ,  Qiannan YU

IPC分类号： G01N33/569 ,  G01N33/543

CPC分类号： G01N33/56983 ,  G01N33/54386 ,  G01N2333/14 ,  G01N2469/20

摘要： This invention provides an ELISA detection kit and method for IgA antibody against Porcine rotavirus group A, belonging to the field of animal husbandry detection technology. The ELISA detection method of this invention utilizes popular PoRV variant strains as parental strains to successfully express recombinant VP6 fusion protein in vitro. Subsequently, the VP6 fusion protein was used as an antigen to be coated onto enzyme-labeled plate to establish a novel ELISA detection method. The method provided by this invention possesses strong sensitivity, high specificity, robust stability, good reproducibility, and ease of preparation, and holds important clinical practical significance for the diagnosis, prevention and control of PoRV.