公开(公告)号：US20090170108A1

公开(公告)日：2009-07-02

申请号：US12315069

申请日：2008-11-26

申请人： Junji Yodoi ,  Norihiko Knodo ,  Aoi Son ,  Noriko Kato ,  Tornijiro Hara ,  Tomohisa Horibe

发明人： Junji Yodoi ,  Norihiko Knodo ,  Aoi Son ,  Noriko Kato ,  Tornijiro Hara ,  Tomohisa Horibe

IPC分类号： C12Q1/68 ,  G01N33/573 ,  C07C203/00 ,  C12N9/02 ,  C12N15/53 ,  C07D307/93

CPC分类号： A61K31/26 ,  A23L33/17 ,  A61K38/44 ,  C12N9/0036 ,  C12Y108/01008

摘要： (Problems) The main object of the present invention is to provide a screening method for selecting a substance affecting a bond between thioredoxin and MIF.(Means for Solving Problems) The present invention provides a screening method for selecting a test substance which strengthens a bond between a polypeptide of a thioredoxin family and a macrophage migration inhibition factor, comprising: mixing a test substance with at least one binding substance selected from following (1) to (4), (1) the polypeptide belonging to the thioredoxin family, (2) a protein having an amino acid sequence of the polypeptide belonging to the thioredoxin family in which one or more amino acid is deleted, replaced or added, and having an equivalent activity to the polypeptide of the thioredoxin family, (3) a gene coding (1), (4) a gene coding (2); bonding the binding substance to the macrophage migration inhibition factor; and monitoring the bond state between the binding substance and the macrophage migration inhibition factor.

摘要翻译： （问题）本发明的主要目的是提供一种选择影响硫氧还蛋白与MIF之间的键的物质的筛选方法。 （解决问题的手段）本发明提供一种筛选方法，用于选择加强硫氧还蛋白家族的多肽与巨噬细胞迁移抑制因子之间的键的检测物质，其包括：将检测物质与至少一种选自 （1）至（4），（1）属于硫氧还蛋白家族的多肽，（2）具有缺失一个或多个氨基酸的硫氧还蛋白家族的多肽的氨基酸序列的蛋白质被替换或 并且具有与硫氧还蛋白家族的多肽相当的活性，（3）编码（1），（4）编码基因（2）的基因; 将结合物质结合至巨噬细胞迁移抑制因子; 并监测结合物质与巨噬细胞迁移抑制因子之间的键状态。