公开(公告)号：US20090170108A1

公开(公告)日：2009-07-02

申请号：US12315069

申请日：2008-11-26

申请人： Junji Yodoi ,  Norihiko Knodo ,  Aoi Son ,  Noriko Kato ,  Tornijiro Hara ,  Tomohisa Horibe

发明人： Junji Yodoi ,  Norihiko Knodo ,  Aoi Son ,  Noriko Kato ,  Tornijiro Hara ,  Tomohisa Horibe

IPC分类号： C12Q1/68 ,  G01N33/573 ,  C07C203/00 ,  C12N9/02 ,  C12N15/53 ,  C07D307/93

CPC分类号： A61K31/26 ,  A23L33/17 ,  A61K38/44 ,  C12N9/0036 ,  C12Y108/01008

摘要： (Problems) The main object of the present invention is to provide a screening method for selecting a substance affecting a bond between thioredoxin and MIF.(Means for Solving Problems) The present invention provides a screening method for selecting a test substance which strengthens a bond between a polypeptide of a thioredoxin family and a macrophage migration inhibition factor, comprising: mixing a test substance with at least one binding substance selected from following (1) to (4), (1) the polypeptide belonging to the thioredoxin family, (2) a protein having an amino acid sequence of the polypeptide belonging to the thioredoxin family in which one or more amino acid is deleted, replaced or added, and having an equivalent activity to the polypeptide of the thioredoxin family, (3) a gene coding (1), (4) a gene coding (2); bonding the binding substance to the macrophage migration inhibition factor; and monitoring the bond state between the binding substance and the macrophage migration inhibition factor.

摘要翻译： （问题）本发明的主要目的是提供一种选择影响硫氧还蛋白与MIF之间的键的物质的筛选方法。 （解决问题的手段）本发明提供一种筛选方法，用于选择加强硫氧还蛋白家族的多肽与巨噬细胞迁移抑制因子之间的键的检测物质，其包括：将检测物质与至少一种选自 （1）至（4），（1）属于硫氧还蛋白家族的多肽，（2）具有缺失一个或多个氨基酸的硫氧还蛋白家族的多肽的氨基酸序列的蛋白质被替换或 并且具有与硫氧还蛋白家族的多肽相当的活性，（3）编码（1），（4）编码基因（2）的基因; 将结合物质结合至巨噬细胞迁移抑制因子; 并监测结合物质与巨噬细胞迁移抑制因子之间的键状态。

公开(公告)号：US20070224150A1

公开(公告)日：2007-09-27

申请号：US11385200

申请日：2006-03-21

申请人： Yongji Chung

发明人： Yongji Chung

IPC分类号： A61K38/18 ,  A61K8/64

CPC分类号： A61K8/64 ,  A61K36/185 ,  A61K38/168 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/2066 ,  A61K38/30 ,  A61K38/44 ,  A61Q7/00 ,  A61Q19/00 ,  A61Q19/08 ,  C12Y108/01008 ,  A61K2300/00

摘要： The present invention relates to a method of treatment for slowing the progress of skin aging comprising contacting the skin with an amount effective to slow skin aging of a composition comprising one or more compounds selected from the group consisting of EGF, bFGF, IGF-1, KGF, TGF-β3, TRX, VEGF, TRX, aFGF, FGF-10, copper peptide, acetyl hexapeptide, palmitoyl pentapeptide, CPP, and UDN glycoprotein.

摘要翻译： 本发明涉及一种用于减缓皮肤老化进程的治疗方法，包括使皮肤接触有效减缓包含一种或多种选自EGF，bFGF，IGF-1， KGF，TGF-β3，TRX，VEGF，TRX，aFGF，FGF-10，铜肽，乙酰六肽，棕榈酰五肽，CPP和UDN糖蛋白。

公开(公告)号：US06838113B1

公开(公告)日：2005-01-04

申请号：US09296927

申请日：1999-04-22

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

IPC分类号： A21D2/26 ,  A21D2/36 ,  A21D8/04 ,  A21D13/06 ,  A23C9/12 ,  A23C9/152 ,  A23C9/20 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/16 ,  A23J3/18 ,  A23K1/16 ,  A23L1/015 ,  A23L1/16 ,  A23L1/182 ,  A23L1/211 ,  A23L1/305 ,  A23L1/314 ,  A61K38/44 ,  A61K39/35 ,  C07K1/113 ,  C07K14/415 ,  C07K14/435 ,  C07K14/46 ,  C07K14/76 ,  C07K14/81 ,  C12N9/02 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  A23L1/29 ,  A23L3/358

CPC分类号： A21D13/064 ,  A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K38/44 ,  A61K39/35 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  A61K2300/00

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

公开(公告)号：US5952034A

公开(公告)日：1999-09-14

申请号：US953703

申请日：1997-10-17

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

IPC分类号： A21D2/26 ,  A21D2/36 ,  A21D8/04 ,  A21D13/06 ,  A23C9/12 ,  A23C9/152 ,  A23C9/20 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/16 ,  A23J3/18 ,  A23K1/16 ,  A23L1/015 ,  A23L1/16 ,  A23L1/182 ,  A23L1/211 ,  A23L1/305 ,  A23L1/314 ,  A61K38/44 ,  A61K39/35 ,  C07K1/113 ,  C07K14/415 ,  C07K14/435 ,  C07K14/46 ,  C07K14/76 ,  C07K14/81 ,  C12N9/02 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  A23L1/03

CPC分类号： A21D13/064 ,  A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A61K38/44 ,  A61K39/35 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S--S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S--S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

摘要翻译： 硫氧还蛋白，一种小二硫醇蛋白，是主要的食品蛋白质，过敏原蛋白质，特别是来自动物和植物来源广泛使用的食品中的过敏原蛋白质的特异性还原剂。 所有靶向蛋白含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。 蛋白质是过敏性活性的，在氧化（S-S）状态下消化不良。 当减少（SH状态）时，它们失去过敏原性和/或变得更易消化。 当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或二硫苏糖醇（化学还原剂）活化（还原）时，硫氧还蛋白实现了这种还原。 用敏化狗进行的皮肤试验和喂养实验表明，在摄取之前用硫氧还蛋白降低的食物处理消除或降低了食物的过敏原性。 研究显示硫氧还蛋白还原后胃蛋白酶和胰蛋白酶消化食物和食物蛋白质。

公开(公告)号：US5792506A

公开(公告)日：1998-08-11

申请号：US326976

申请日：1994-10-21

申请人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee ,  Rosa Lozano ,  Oscar L. Frick ,  Richard W. Ermel

发明人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee ,  Rosa Lozano ,  Oscar L. Frick ,  Richard W. Ermel

IPC分类号： A23K1/18 ,  A21D2/26 ,  A21D2/36 ,  A21D8/04 ,  A21D13/06 ,  A23C9/00 ,  A23C9/12 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K1/16 ,  A23L1/015 ,  A23L1/10 ,  A23L1/16 ,  A23L1/182 ,  A23L1/20 ,  A23L1/211 ,  A23L1/305 ,  A23L1/31 ,  A23L1/314 ,  A61K35/20 ,  A61K35/34 ,  A61K35/54 ,  A61K36/899 ,  A61K38/44 ,  A61K38/46 ,  A61K39/35 ,  A61P37/08 ,  C07K1/00 ,  C07K1/113 ,  C07K14/415 ,  C07K14/435 ,  C07K14/46 ,  C07K14/76 ,  C07K14/81 ,  C12N9/02 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  A23L1/03

CPC分类号： A21D13/064 ,  A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A61K39/35 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for major allergenic proteins present in widely used foods from animal and plant sources. All targeted allergenic proteins contain disulfide (S--S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active in the oxidized (S--S) state. When reduced (SH state), they lose their allergenicity. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food.

摘要翻译： 硫氧还蛋白，小二硫醇蛋白，是来自动物和植物来源的广泛使用的食品中存在的主要变应原蛋白的特异性还原剂。 所有靶向过敏原蛋白都含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。 蛋白质在氧化（S-S）状态下具有过敏性活性。 当减少（SH状态）时，它们失去其过敏原性。 当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或二硫苏糖醇（化学还原剂）活化（还原）时，硫氧还蛋白实现了这种还原。 用敏化狗进行的皮肤试验和喂养实验表明，在摄取之前用硫氧还蛋白降低的食物处理消除或降低了食物的过敏原性。

公开(公告)号：US5460810A

公开(公告)日：1995-10-24

申请号：US941372

申请日：1992-09-02

申请人： David A. Williams ,  Steven C. Clark

发明人： David A. Williams ,  Steven C. Clark

IPC分类号： A61K38/00 ,  A61K38/18 ,  A61K38/20 ,  A61P1/00 ,  A61P43/00 ,  C07K14/54 ,  C12N9/02 ,  A61K38/19

CPC分类号： C07K14/5431 ,  A61K38/204 ,  C12N9/0036 ,  C12Y108/01008 ,  A61K38/00 ,  Y10S514/867 ,  Y10S514/908

摘要： A method for reducing damage or depletion of gut epithelial cells (e.g., as a result of radiation therapy or chemotherapy) by administration of one or more of the following cytokines: interleukin 11 (IL-11), interleukin 6 (IL-6), leukemia inhibitory factor/cholinergic differentiation factor (LIF/CDF), oncostatin M (OSM), or ciliary neurotrophic factor (CNTF).

摘要翻译： 通过施用以下一种或多种细胞因子来减少肠上皮细胞损伤或消耗的方法（例如，作为放射治疗或化疗的结果）：白细胞介素11（IL-11），白介素6（IL-6）， 白血病抑制因子/胆碱能分化因子（LIF / CDF），制瘤素M（OSM）或睫状神经营养因子（CNTF）。

公开(公告)号：US07253144B2

公开(公告)日：2007-08-07

申请号：US10992360

申请日：2004-11-19

申请人： Mikako Shirouzu ,  Goushi Ishihara ,  Mihoro Saeki ,  Mie Goto ,  Kaori Tajima ,  Takanori Kigawa ,  Shigeyuki Yokoyama

发明人： Mikako Shirouzu ,  Goushi Ishihara ,  Mihoro Saeki ,  Mie Goto ,  Kaori Tajima ,  Takanori Kigawa ,  Shigeyuki Yokoyama

IPC分类号： C12N15/70 ,  C12N15/63 ,  C07K14/705 ,  C07K21/04 ,  A61K38/00

CPC分类号： C12N15/62 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/033 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/35 ,  C07K2319/50 ,  C12N9/0036 ,  C12P21/00 ,  C12Y108/01008

摘要： We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.

摘要翻译： 我们要求一种表达硫氧还蛋白融合膜蛋白的方法，包括在非离子型洗涤剂存在下，在无细胞蛋白质合成系统中表达所述硫氧还蛋白融合的膜蛋白。 作为高度疏水性的蛋白质，例如G蛋白偶联受体的融合膜蛋白质，优选可以高度溶解，能够形成生物活性的三维结构体。 此外，本发明提供编码在无细胞蛋白质合成系统中表达的硫氧还蛋白融合膜蛋白的重组多核苷酸。

公开(公告)号：US07074900B2

公开(公告)日：2006-07-11

申请号：US10422422

申请日：2003-04-23

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

IPC分类号： C07K14/00 ,  C07K14/415 ,  A61K39/35 ,  A61K39/36

CPC分类号： A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y10S436/904 ,  Y10T436/182

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

公开(公告)号：US5646016A

公开(公告)日：1997-07-08

申请号：US165301

申请日：1993-12-10

申请人： John McCoy ,  Elizabeth DiBlasio-Smith ,  Kathleen Grant ,  Edward R. LaVallie

发明人： John McCoy ,  Elizabeth DiBlasio-Smith ,  Kathleen Grant ,  Edward R. LaVallie

IPC分类号： C07K14/00 ,  C07K14/475 ,  C07K14/51 ,  C07K14/52 ,  C07K14/53 ,  C07K14/54 ,  C07K14/55 ,  C12N1/06 ,  C12N9/02 ,  C12N15/24 ,  C12N15/62 ,  C07K1/22 ,  C12N1/00 ,  C12N15/63

CPC分类号： C12N9/0036 ,  C07K14/00 ,  C07K14/475 ,  C07K14/51 ,  C07K14/52 ,  C07K14/523 ,  C07K14/53 ,  C07K14/54 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5431 ,  C07K14/55 ,  C12N1/066 ,  C12N15/62 ,  C12Y108/01008 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/20 ,  C07K2319/35 ,  C07K2319/75

摘要： Provided is a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to a DNA sequence encoding a second peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. The fusion molecule may be modified to introduce one or more metal-binding/chelating amino-acid residues to aid in purification. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.

摘要翻译： 提供了包含编码与编码第二肽或蛋白质的DNA序列融合的硫氧还蛋白样蛋白质的DNA序列的融合分子。 肽或蛋白质可以融合到硫氧还蛋白样分子的氨基末端，硫氧还蛋白样分子的羧基末端，或硫氧还蛋白样分子内，例如在所述分子的活性位点环上。 可以修饰融合分子以引入一种或多种金属结合/螯合氨基酸残基以有助于纯化。 在能够将其表达在所需宿主细胞中的调节序列控制下的该融合分子的表达产生高水平的稳定和可溶性融合蛋白。 位于细菌细胞质中的融合蛋白可通过渗透休克或冷冻/解冻程序从细胞中选择性释放。 可以任选地切割以从硫氧还蛋白样部分释放可溶性的，正确折叠的异源蛋白质。

公开(公告)号：US5635182A

公开(公告)日：1997-06-03

申请号：US260582

申请日：1994-06-16

申请人： John M. McCoy ,  Zhijian Lu

发明人： John M. McCoy ,  Zhijian Lu

IPC分类号： A61K39/00 ,  C07K14/245 ,  C12N9/02 ,  C12N15/62 ,  G01N33/68 ,  C12N15/00

CPC分类号： C12N9/0036 ,  C07K14/245 ,  C12N15/62 ,  C12Y108/01008 ,  G01N33/68 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/035 ,  C07K2319/35

摘要： Provided by the present invention are novel methods of detecting ligand interactions, as well as regents useful in the method, including DNA and host cells; and more specifically relates to novel methods for the detection of protein/protein interactions and their application in epitope mapping and the study of ligand/receptor interactions. Also provided are vaccines and kits comprising the expression products and host cells of the invention.

摘要翻译： 本发明提供了检测配体相互作用的新方法，以及在该方法中有用的试剂，包括DNA和宿主细胞; 更具体地涉及用于检测蛋白质/蛋白质相互作用的新方法及其在表位作图中的应用以及配体/受体相互作用的研究。 还提供了包含本发明的表达产物和宿主细胞的疫苗和试剂盒。