公开(公告)号：US07056682B2

公开(公告)日：2006-06-06

申请号：US10474755

申请日：2002-12-27

申请人： Akihito Kamei ,  Noriko Kenjo ,  Tatsuro Kawamura ,  Mahito Hirai

发明人： Akihito Kamei ,  Noriko Kenjo ,  Tatsuro Kawamura ,  Mahito Hirai

IPC分类号： G01N33/53

CPC分类号： G01N33/5375 ,  G01N33/536 ,  G01N33/539 ,  G01N33/542

摘要： In step St1 shown in FIG. 1, a reaction system including a sample for which the content of a subject substance is to be measured, a specific-binding substance specifically binding to the subject substance, and phthalic acid or phthalate salt is constructed. The pH of the reaction system is set at less than 7. When the subject substance is an antigen, the specific-binding substance is an antibody. In reverse, when the subject substance is an antibody, the specific-binding substance is an antigen. In step St2 in FIG. 1, an optical property of the reaction system is measured. When agglutinate complexes are produced in the step St1, the reaction system becomes turbid, causing a change in scattered light intensity, transmitted light amount and the like. Therefore, by measuring the scattered light intensity, the transmitted light amount or the like, the degree of turbidity of the reaction system can be estimated.

摘要翻译： 在图1所示的步骤St 1中， 如图1所示，构成了包含被测定物质含量的样品，特异性结合物质的特异性结合物和邻苯二甲酸或邻苯二甲酸盐的反应体系。 反应体系的pH设定为小于7.当目标物质为抗原时，特异性结合物质为抗体。 相反，当目标物质是抗体时，特异性结合物质是抗原。 在图3的步骤St2中 如图1所示，测定反应体系的光学特性。 当在步骤St 1中产生凝集络合物时，反应体系变得混浊，导致散射光强度，透射光量等的变化。 因此，通过测量散射光强度，透射光量等，可以估计反应体系的浊度。