METHOD FOR EMBEDDING A BIOLOGICAL SAMPLE IN A TRANSPARENT MATRIX FOR ANALYSIS USING SINGLE PLANE ILLUMINATION MICROSCOPY
    1.
    发明申请
    METHOD FOR EMBEDDING A BIOLOGICAL SAMPLE IN A TRANSPARENT MATRIX FOR ANALYSIS USING SINGLE PLANE ILLUMINATION MICROSCOPY 审中-公开
    用于使用单平面照射显微镜进行分析的透明矩阵中嵌入生物样品的方法

    公开(公告)号:US20110094318A1

    公开(公告)日:2011-04-28

    申请号:US12997113

    申请日:2009-06-06

    IPC分类号: G01N1/28

    CPC分类号: G01N1/28 G02B21/32 G02B21/34

    摘要: The invention is directed to method for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement. According to the invention, the method mentioned above has the following method steps: a sample is introduced into a transparent medium, preferably agarose gel, which is initially liquid; the medium is changed from the liquid state to the solid state, wherein the sample is fixated within the medium, but the transparency of the medium is retained; the solidified medium is positioned in the microscope arrangement in such a way that the sample enclosed therein is situated in the detection area of the objective. Further, a device is proposed for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement.

    摘要翻译: 本发明涉及在显微镜装置的目标的检测区域中定位和对准优选生物样品的方法。 根据本发明,上述方法具有以下方法步骤:将样品引入最初为液体的透明介质,优选琼脂糖凝胶; 介质从液态变为固态,其中样品固定在介质中,但保持介质的透明度; 固化介质以这样一种方式定位在显微镜装置中,使得封闭在其中的样品位于物镜的检测区域中。 此外,提出了一种用于在显微镜装置的目标的检测区域中定位和对准优选生物样品的装置。