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    Development of a PCR-based method for identification of Tilletia indica, causal agent of Karnal bunt of wheat
    1.
    发明授权
    Development of a PCR-based method for identification of Tilletia indica, causal agent of Karnal bunt of wheat 失效
    开发了一种基于PCR的方法，用于鉴定小麦K of的致病因子

    公开(公告)号：US5776686A

    公开(公告)日：1998-07-07

    申请号：US772961

    申请日：1996-12-24

    申请人： Oney P. Smith ,  Gary L. Peterson ,  Raymond J. Beck ,  Morris R. Bonde ,  Norman W. Schaad

    发明人： Oney P. Smith ,  Gary L. Peterson ,  Raymond J. Beck ,  Morris R. Bonde ,  Norman W. Schaad

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12Q1/6895

    摘要： The polymerase chain reaction (PCR) was used to identify Tilletia indica, the causal agent of Karnal bunt of wheat. The method uses two sets of oligonucleotide primers developed by sequence analysis of cloned Dra I fragments of mitochondrial DNA of T. indica. The primer pair TI17M1 (5'-TCCCCTTGGATCAGAACGTA-3') and TI17M2 (5'-AGAAGTCTAACTCCCCCCTCT-3'), derived from clone pTI-MD17, amplified a single 825-bp product from all isolates of T. indica and no products for other Tilletia species. In addition, the primer pair TI57M1 (5'-TTTTCCCTCTCTC-CTTTTTTCA-3') and TI57M2 (5'-AGCAAAGACAAAGTAGGCTTCC-3'), derived from clone pTI-MD57, produced a product of 118 bp which was unique to T. indica.

    摘要翻译： 使用聚合酶链反应（PCR）来鉴定小麦的Karnal bunt的致病剂Tilletia indica。 该方法采用两组寡核苷酸引物，通过对indic of线粒体DNA的克隆DraⅠ片段进行序列分析而开发。 衍生自克隆pTI-MD17的引物对TI17M1（5'-TCCCCTTGGATCAGAACGTA-3'）和TI17M2（5'-AGAAGTCTAACTCCCCCCTCT-3'）扩增了来自indic from的所有分离株的单个825bp产物， 其他Tilletia种。 另外，来自克隆pTI-MD57的引物对TI57M1（5'-TTTTCCCTCTCTC-CTTTTTTCA-3'）和TI57M2（5'-AGCAAAGACAAAGTAGGCTTCC-3'）产生118bp的产物，其是T. indica 。