公开(公告)号：US5041378A

公开(公告)日：1991-08-20

申请号：US84479

申请日：1987-08-11

申请人： Robert J. Drummond ,  Will Bloch ,  Brian W. Matthews ,  Pamela L. Toy

发明人： Robert J. Drummond ,  Will Bloch ,  Brian W. Matthews ,  Pamela L. Toy

IPC分类号： C12N9/36 ,  C12N9/92 ,  C12P21/02

CPC分类号： C12N9/2462 ,  C12N9/92 ,  C12P21/02 ,  Y10S435/886

摘要： Xylose isomerase (XI) muteins useful in the conversion of glucose to fructose or xylose to xylulose are obtained in usable amounts by protein structural and recombinant DNA methods, including x-ray crystallography, cloning, computer graphic modeling and site-directed mutagenesis and expression of the bacterial DNA sequences encoding native procaryotic xylose isomerase. These native sequences are altered to encode the xylose isomerase muteins having improved catalytic function and/or thermostability.

摘要翻译： 通过蛋白质结构和重组DNA方法获得可用于将葡萄糖转化为果糖或木糖至木酮糖的木糖异构酶（XI）突变蛋白，包括x射线晶体学，克隆，计算机图形建模和定点诱变和表达 编码天然原核木糖异构酶的细菌DNA序列。 改变这些天然序列以编码具有改善的催化功能和/或热稳定性的木糖异构酶突变蛋白。