公开(公告)号：US06406841B1

公开(公告)日：2002-06-18

申请号：US08259451

申请日：1994-06-20

申请人： Helen H. Lee ,  Priscilla A. Swanson ,  Kenneth B. Idler ,  Joseph D. Rosenblatt ,  Irvin S. Y. Chen ,  David W. Golde ,  Eugene Robertson ,  John E. Stephens ,  Emerson W. Chan ,  Mark H. Buytendorp ,  Joan E. Johnson ,  Cheryl T. Motley ,  Michelle Edwards ,  Cynthia Tate ,  Bryan Peterson ,  Peggy Guidinger

发明人： Helen H. Lee ,  Priscilla A. Swanson ,  Kenneth B. Idler ,  Joseph D. Rosenblatt ,  Irvin S. Y. Chen ,  David W. Golde ,  Eugene Robertson ,  John E. Stephens ,  Emerson W. Chan ,  Mark H. Buytendorp ,  Joan E. Johnson ,  Cheryl T. Motley ,  Michelle Edwards ,  Cynthia Tate ,  Bryan Peterson ,  Peggy Guidinger

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2740/14022 ,  G01N33/56988

摘要： The present invention relates generally to a novel human T-cell lymphotropic, or leukemia, virus type II (HTLV-II) isolate designated NRA. HTLV-IINRA was originally isolated from a patient with atypical hairy cell leukemia. Preliminary restriction analysis of this isolate demonstrated that it differs genetically from the prototypical HTLV-II isolate Mo. HTLV-IINRA proviral molecular clones were obtained and the entire nucleotide sequence of the virus ascertained. The claimed invention is particularly directed toward the gp46 and p21e envelope proteins encoded by the env gene. Methods and kits for the detection of HTLV-II antibodies employing these envelope proteins are also described.

摘要翻译： 本发明一般涉及一种命名为NRA的新型人类T细胞淋巴细胞性或白血病II型病毒（HTLV-II）分离物。 HTLV-IINRA最初是从非典型毛细胞白血病患者分离出来的。 该分离株的初步限制性分析表明，它与遗传上与原型HTLV-II分离株Mo不同，获得HTLV-IINRA前病毒分子克隆，确定病毒的全部核苷酸序列。 所要求保护的发明特别针对由env基因编码的gp46和p21e包膜蛋白。 还描述了使用这些包膜蛋白检测HTLV-II抗体的方法和试剂盒。