公开(公告)号：US09057095B1

公开(公告)日：2015-06-16

申请号：US13350405

申请日：2012-01-13

Applicant: Melissa Tuchscherer ,  Perry Kilpatrick ,  Richard Slawecki ,  Fernando Manuel Rubio

Inventor： Melissa Tuchscherer ,  Perry Kilpatrick ,  Richard Slawecki ,  Fernando Manuel Rubio

IPC: C12Q1/46 ,  C12Q1/34 ,  G01N31/22 ,  G01N33/573

CPC classification number: C12Q1/46 ,  C12Q2334/00 ,  G01N21/78 ,  G01N31/22

Abstract: The present invention provides a kit and a method of testing for carbamates in amine cured epoxies. The method and kit are based on the use of an enzyme that is inhibited by carbamates. A specific embodiment of the kit includes acetylthiocholine (ATC), dithio-bis (2-nitrobenzoic acid) (DTNB), and acetyl-cholinesterase (ACh-E). In the corresponding method embodiment, the method comprises the steps of collecting carbamates from the epoxy, adding the collected carbamates to a solution, adding acetyl-cholinesterase (ACh-E) to the solution, adding acetylthiocholine (ATC) to the solution, adding dithio-bis (2-nitrobenzoic acid) (DTNB) to the solution, and ultimately measuring the intensity of the yellow color of the solution.

Abstract translation: 本发明提供了胺固化环氧化物中氨基甲酸酯的试剂盒和方法。 该方法和试剂盒基于使用被氨基甲酸酯抑制的酶。 试剂盒的具体实施方案包括乙酰硫胆碱（ATC），二硫代双（2-硝基苯甲酸）（DTNB）和乙酰胆碱酯酶（ACh-E）。 在相应的方法实施方案中，该方法包括以下步骤：从环氧树脂中收集氨基甲酸酯，将收集的氨基甲酸酯加入到溶液中，向溶液中加入乙酰胆碱酯酶（ACh-E），向溶液中加入乙酰硫胆碱（ATC），加入二硫 双（2-硝基苯甲酸）（DTNB）溶液，最终测定溶液的黄色强度。

公开(公告)号：US06261631B1

公开(公告)日：2001-07-17

申请号：US09469328

申请日：1999-12-22

Applicant: Henry Lomasney ,  Perry Kilpatrick ,  Shirish Pandit

Inventor： Henry Lomasney ,  Perry Kilpatrick ,  Shirish Pandit

IPC: B05D138

CPC classification number: C09D7/00 ,  B05D5/061 ,  B05D7/53

Abstract: A method for controlling uniformity of applied film thickness of clear protective coatings by means of a color-dissipatable dye additive which dissipates within a reasonable timeframe, generally from hours to a few days, leaving the applied film with no evidence of its use during the application phase. The color additives temporarily impart color to an otherwise clear coating, and subsequently dissipate over a short period of time. An embodiment of this technology includes the addition of a compatibilizer carrier solution to increase the ease of measuring the dye for addition to the clear coating.

Abstract translation: 一种通过在合理时间范围内消散的彩色可消散染料添加剂来控制透明保护涂层的涂膜均匀性的方法，通常为数小时至数天，使得涂布膜在使用过程中没有使用证据 相。 颜色添加剂暂时将颜色赋予其他透明涂层，随后在短时间内消散。 该技术的一个实施方案包括添加增容剂载体溶液以增加测量染料以便添加到透明涂层中的容易程度。

公开(公告)号：US06488719B2

公开(公告)日：2002-12-03

申请号：US09852152

申请日：2001-05-10

Applicant: Henry Lomasney ,  Perry Kilpatrick ,  Shirish Pandit

Inventor： Henry Lomasney ,  Perry Kilpatrick ,  Shirish Pandit

IPC: D06P513

CPC classification number: C09D7/00 ,  B05D5/061 ,  B05D7/53

Abstract: A method for controlling uniformity of applied film thickness of clear protective coatings by means of a color-dissipatable dye additive which dissipates within a reasonable timeframe, generally from hours to a few days, leaving the applied film with no evidence of its use during the application phase. The color additives temporarily impart color to an otherwise clear coating, and subsequently dissipate over a short period of time. An embodiment of this technology includes the addition of a compatibilizer carrier solution to increase the ease of measuring the dye for addition to the clear coating.

Abstract translation: 一种通过在合理时间范围内消散的彩色可消散染料添加剂来控制透明保护涂层的涂膜均匀性的方法，通常为数小时至数天，使得涂布膜在使用过程中没有使用证据 相。 颜色添加剂暂时将颜色赋予其他透明涂层，随后在短时间内消散。 该技术的一个实施方案包括添加增容剂载体溶液以增加测量染料以便添加到透明涂层中的容易程度。