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1.发明申请Spatial Biomarker of Disease and Detection of Spatial Organization of Cellular Receptors 有权疾病空间生物标志物和细胞受体空间组织检测公开(公告)号：US20120178104A1
公开(公告)日：2012-07-12
申请号：US13194577
申请日：2011-07-29
IPC分类号： G01N21/75
CPC分类号： C12Q1/6886 , C12Q2600/136 , C12Q2600/156
摘要： A signature of a condition of a live cell is established in an assay that allows distribution of the receptors on the cell surface in response to binding a ligand. The receptors can be optically detected and quantified to provide a value for the condition, Test drugs can be screened for therapeutic potential in the assay: a potentially efficacious drug is identified by an ability to modulate an established signature. The receptor distribution signature can be corroborated with an mRNA expression profile of several genes, indicating, for example, metastasis.
摘要翻译： 在允许响应于结合配体的细胞表面上分配受体的测定中建立活细胞条件的标记。 可以光学检测和定量受体以提供该病症的价值。在测定中可以筛选测试药物的治疗潜力：通过调节已建立的特征的能力鉴定潜在有效的药物。 受体分布特征可以用几种基因的mRNA表达谱进行证实，表明例如转移。
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2.发明申请Fluid Membrane-Based Ligand Display System for Live Cell Assays and Disease Diagnosis Applications 审中-公开用于活细胞分析和疾病诊断应用的流体膜基配体显示系统公开(公告)号：US20090011428A1
公开(公告)日：2009-01-08
申请号：US12161553
申请日：2007-01-18
申请人： Jwa-Min Nam , Pradeep M. Nair , Richard M. Neve , Joe W. Gray , John T. Groves
发明人： Jwa-Min Nam , Pradeep M. Nair , Richard M. Neve , Joe W. Gray , John T. Groves
IPC分类号： G01N33/53
CPC分类号： G01N33/554
摘要： A supported membrane based, strategy for the presentation of soluble signaling molecules to living cells is described. In this system, the fluidity of the supported membrane enables localized enrichment of ligand density in a configuration reflecting cognate receptor distribution on the cell surface. Display of a ligand in non-fluid supported membranes produces significantly less cell adhesion and spreading, thus demonstrating that this technique provides a means to control functional soluble ligand exposure in a surface array format. Furthermore, this technique can be applied to tether natively membrane-bound signaling molecules such as ephrin A1 to a supported lipid bilayer. Such a surface can modulate the spreading behavior of metastatic human breast cancer cells displaying ligands and biomolecules of choice. The SLB microenvironment provides a versatile platform that can be tailored to controllably and functionally present a multitude of cell signaling events in a parallel surface array format.
摘要翻译： 描述了一种用于向活细胞呈递可溶性信号分子的支持膜的策略。 在该系统中，支持的膜的流动性使得能够在反映细胞表面上的同源受体分布的构型中局部富集配体密度。 在非流体支持的膜中显示配体产生显着更少的细胞粘附和扩散，因此证明该技术提供了以表面阵列形式控制功能性可溶性配体暴露的方法。 此外，该技术可以应用于系统地将膜结合的信号分子例如ephrin A1应用于支持的脂质双层。 这种表面可以调节显示配体和选择的生物分子的转移性人乳腺癌细胞的扩散行为。 SLB微环境提供了一个通用平台，可以进行调整，以便可控地和功能地呈现并行表面阵列格式的多个单元信令事件。
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公开(公告)号：US09708665B2
公开(公告)日：2017-07-18
申请号：US13194577
申请日：2011-07-29
CPC分类号： C12Q1/6886 , C12Q2600/136 , C12Q2600/156
摘要： A signature of a condition of a live cell is established in an assay that allows distribution of the receptors on the cell surface in response to binding a ligand. The receptors can be optically detected and quantified to provide a value for the condition, Test drugs can be screened for therapeutic potential in the assay: a potentially efficacious drug is identified by an ability to modulate an established signature. The receptor distribution signature can be corroborated with an mRNA expression profile of several genes, indicating, for example, metastasis.
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