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    Plasmonic System for Detecting Binding of Biological Molecules
    1.
    发明申请
    Plasmonic System for Detecting Binding of Biological Molecules 审中-公开
    用于检测生物分子结合的等离子体系统

    公开(公告)号：US20120208174A1

    公开(公告)日：2012-08-16

    申请号：US13204506

    申请日：2011-08-05

    Applicant: William J. Galush ,  Sarah A. Shelby ,  Martin J. Mulvihill ,  Andrea R. Tao ,  Peidong Yang ,  John T. Groves

    Inventor： William J. Galush ,  Sarah A. Shelby ,  Martin J. Mulvihill ,  Andrea R. Tao ,  Peidong Yang ,  John T. Groves

    IPC: G01N21/75 ,  G01N21/47 ,  G01N21/59 ,  G01N21/55 ,  B82Y15/00

    CPC classification number: G01N33/54346 ,  G01N21/554

    Abstract: Detection and characterization of molecular interactions on membrane surfaces is important to biological and pharmacological research. In one embodiment, silver nanocubes interfaced with glass-supported model membranes form a label-free sensor that measures protein binding to the membrane. The present device and technique utilizes plasmon resonance scattering of nanoparticles, which are chemically coupled to the membrane. In contrast to other plasmonic sensing techniques, this method features simple, solution-based device fabrication and readout. Static and dynamic protein/membrane binding are monitored and quantified.

    Abstract translation: 膜表面分子相互作用的检测和表征对生物学和药理学研究很重要。 在一个实施方案中，与玻璃支持的模型膜相接合的银纳米管形成测量蛋白质结合膜的无标记传感器。 本发明的装置和技术利用等离子体共振散射纳米颗粒，其与化学偶联。 与其他等离子体感测技术相比，该方法具有简单的基于解决方案的器件制造和读出。 静态和动态蛋白/膜结合被监测和定量。

    Fluid Membrane-Based Ligand Display System for Live Cell Assays and Disease Diagnosis Applications
    3.
    发明申请
    Fluid Membrane-Based Ligand Display System for Live Cell Assays and Disease Diagnosis Applications 审中-公开
    用于活细胞分析和疾病诊断应用的流体膜基配体显示系统

    公开(公告)号：US20090011428A1

    公开(公告)日：2009-01-08

    申请号：US12161553

    申请日：2007-01-18

    Applicant: Jwa-Min Nam ,  Pradeep M. Nair ,  Richard M. Neve ,  Joe W. Gray ,  John T. Groves

    Inventor： Jwa-Min Nam ,  Pradeep M. Nair ,  Richard M. Neve ,  Joe W. Gray ,  John T. Groves

    IPC: G01N33/53

    CPC classification number: G01N33/554

    Abstract: A supported membrane based, strategy for the presentation of soluble signaling molecules to living cells is described. In this system, the fluidity of the supported membrane enables localized enrichment of ligand density in a configuration reflecting cognate receptor distribution on the cell surface. Display of a ligand in non-fluid supported membranes produces significantly less cell adhesion and spreading, thus demonstrating that this technique provides a means to control functional soluble ligand exposure in a surface array format. Furthermore, this technique can be applied to tether natively membrane-bound signaling molecules such as ephrin A1 to a supported lipid bilayer. Such a surface can modulate the spreading behavior of metastatic human breast cancer cells displaying ligands and biomolecules of choice. The SLB microenvironment provides a versatile platform that can be tailored to controllably and functionally present a multitude of cell signaling events in a parallel surface array format.

    Abstract translation: 描述了一种用于向活细胞呈递可溶性信号分子的支持膜的策略。 在该系统中，支持的膜的流动性使得能够在反映细胞表面上的同源受体分布的构型中局部富集配体密度。 在非流体支持的膜中显示配体产生显着更少的细胞粘附和扩散，因此证明该技术提供了以表面阵列形式控制功能性可溶性配体暴露的方法。 此外，该技术可以应用于系统地将膜结合的信号分子例如ephrin A1应用于支持的脂质双层。 这种表面可以调节显示配体和选择的生物分子的转移性人乳腺癌细胞的扩散行为。 SLB微环境提供了一个通用平台，可以进行调整，以便可控地和功能地呈现并行表面阵列格式的多个单元信令事件。

    Porphyrin catalysts and methods of use thereof
    6.
    发明授权
    Porphyrin catalysts and methods of use thereof 有权
    卟啉催化剂及其使用方法

    公开(公告)号：US08232267B2

    公开(公告)日：2012-07-31

    申请号：US12311640

    申请日：2007-10-05

    Applicant: John T. Groves

    Inventor： John T. Groves

    IPC: A01N55/02 ,  A01N43/36 ,  A61K31/40 ,  C07B47/00 ,  C07D487/22

    CPC classification number: C07F15/025 ,  C07D487/22 ,  C07F3/003

    Abstract: This invention provides a novel class of substituted macrocyclic porphyrin compounds. The compounds are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, or prodrug thereof are also described.

    Abstract translation: 本发明提供了一类新的取代的大环卟啉化合物。 该化合物可用作过氧亚硝酸盐分解催化剂。 还描述了药物组合物，以及制备和使用该化合物或其药学上可接受的盐，水合物或前药的方法。

    Membrane-based assays
    7.
    发明申请
    Membrane-based assays 审中-公开

    公开(公告)号：US20080176759A1

    公开(公告)日：2008-07-24

    申请号：US11968078

    申请日：2007-12-31

    Applicant: Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    Inventor： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    IPC: C40B30/04

    CPC classification number: C40B30/04 ,  C12Q1/18 ,  G01N33/54373 ,  G01N33/554 ,  G01N2500/00

    Abstract: Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

    Membrane-coated particles
    9.
    发明授权
    Membrane-coated particles 有权
    膜包衣颗粒

    公开(公告)号：US08962344B2

    公开(公告)日：2015-02-24

    申请号：US12922993

    申请日：2009-03-16

    Applicant: John T. Groves ,  Jeremy T. Blitzer

    Inventor： John T. Groves ,  Jeremy T. Blitzer

    IPC: G01N33/543 ,  G01N33/567

    CPC classification number: G01N33/54313 ,  G01N33/567

    Abstract: A membrane-coated particle composition and methods comprising a particle surrounded by a native cell membrane are disclosed. The cell membrane may contain selected receptors or binding components. At least a portion of the receptors or binding components are oriented on the membrane-coated particle in the same or similar orientation as in the native cell membrane. The membrane-coated particle(s) finds use, for example, in contexts of basic research, proteomics, drug discovery, drug delivery, medical diagnostics, and aspects of patient care.

    Abstract translation: 公开了一种膜包覆颗粒组合物和包含由天然细胞膜包围的颗粒的方法。 细胞膜可以含有选择的受体或结合成分。 受体或结合组分的至少一部分以与天然细胞膜相同或相似的取向定向在膜包被的颗粒上。 膜包被的颗粒可用于例如基础研究，蛋白质组学，药物发现，药物递送，医学诊断和患者护理方面的情况。

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