公开(公告)号：US20190195881A1

公开(公告)日：2019-06-27

申请号：US16227642

申请日：2018-12-20

申请人： Proplex Technologies, LLC

发明人： Frank N. Chang ,  George P. Tuszynski ,  Solomon Luo ,  Jeff Yang

IPC分类号： G01N33/574

CPC分类号： G01N33/57492 ,  G01N2333/4719

摘要： A highly sensitive method of detecting GASP-1 or a fragment thereof in a sample is provided. The method comprises (a) exposing a surface to a sample comprising GASP-1 or a fragment thereof; (b) immobilizing an anti-GASP-1 antibody to the surface in the presence of the GASP-1 or a fragment thereof; (c) measuring the amount of the anti-GASP-1 antibody immobilized to the surface; and (d) determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 antibody immobilized to the surface. A coating agent may be immobilized to the surface in step (a), and the anti-GASP-1 antibody may be immobilized to the surface via the coating agent. The coating agent may be selected from the group consisting of a conjugate of bovine serum albumin (BSA) and a GASP-1 peptide (BSA-GASP-1 conjugate) a capture antibody against a microvesicle or exosomal surface biomarker, and a combination thereof. Also provided are kits for detecting GASP-1 or its fragment.

公开(公告)号：US12110340B2

公开(公告)日：2024-10-08

申请号：US16408510

申请日：2019-05-10

申请人： Proplex Technologies, LLC

发明人： Frank N. Chang ,  George P. Tuszynski ,  Solomon Luo ,  Jeff Yang

IPC分类号： C07K16/30 ,  A61P35/00 ,  C07K14/705 ,  C07K14/71 ,  C07K14/725 ,  C12N5/0783 ,  G01N33/574

CPC分类号： C07K16/30 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/70521 ,  C07K14/71 ,  C12N5/0638 ,  G01N33/57488 ,  G01N33/57492 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/02 ,  C07K2319/03

摘要： A method for detecting cells having granules expressing G protein coupled receptor-associated sorting protein 1 (GASP-1) or a fragment thereof is provided. The detection method may comprise: (a) contacting the cells with an effective amount of a binding protein, wherein the binding protein comprises an antigen binding fragment that specifically binds GASP-1; and (b) identifying cells having granules bound to the binding protein. The GASP-1 granules may be in the cytosol or on the surface of the cells. Also provided are methods for producing T-cells comprising a chimeric antigen receptor, anti-GASP-1 antibody or a bi-specific binding protein. Further provided are methods for treating GASP-1-mediated to disease or inactivating exosomes, microvesicles or oncosomes.

公开(公告)号：US20200225235A1

公开(公告)日：2020-07-16

申请号：US16827145

申请日：2020-03-23

申请人： Proplex Technologies, LLC

发明人： Frank N. Chang ,  George P. Tuszynski ,  Solomon Luo ,  Jeff Yang

IPC分类号： G01N33/574 ,  C07K14/72

摘要： A highly sensitive method of detecting GASP-1 or a fragment thereof in a sample is provided. The method comprises (a) exposing a surface to a sample comprising GASP-1 or a fragment thereof; (b) immobilizing an anti-GASP-1 detection antibody to the surface; (c) measuring the amount of the anti-GASP-1 detection antibody immobilized to the surface; and (d) determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface. A coating agent may be immobilized to the surface in step (a), and the anti-GASP-1 detection antibody may be immobilized to the surface via the coating agent, directly or indirectly. The coating agent may be selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein (e.g., bovine serum albumin (BSA)) and a GASP-1 peptide, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. Also provided are kits for detecting GASP-1 or its fragment.