公开(公告)号：US20140369973A1

公开(公告)日：2014-12-18

申请号：US14363748

申请日：2012-12-07

申请人： FRED HUTCHINSON CANCER RESEARCH CENTER ,  NOVARTIS INSTITUTE FOR FUNCTIONAL GENOMICS, INC. DBA THE GENOMICS INSTITUTE OF THE NORVATIS ,  RESEARCH FOUNDATION (GNF)

发明人： Irwin D. Bernstein ,  Anthony E. Boitano ,  Michael Cooke

IPC分类号： C12N5/0789 ,  A61K35/28

CPC分类号： C12N5/0647 ,  A61K35/28 ,  A61K2035/124 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/38 ,  C12N2501/42 ,  C12N2501/60 ,  C12N2501/999

摘要： The present invention relates to methods, kits and compositions for expansion of hematopoietic stem/progenitor cells and providing hematopoietic function to human patients in need thereof. In one aspect, it relates to kits and compositions comprising a Notch agonist and an aryl hydrocarbon receptor antagonist. Also provided herein are methods for expanding the hematopoietic stem/progenitor cells using kits and compositions comprising a Notch agonist and an aryl hydrocarbon receptor antagonist. The hematopoietic stem/progenitor cells expanded using the disclosed kits, compositions and methods include human umbilical cord blood stem/progenitor cells, placental cord blood stem/progenitor cells and peripheral blood stem cells. The present invention also relates to administering hematopoietic stem/progenitor cells expanded using a combination of a Notch agonist and an aryl hydrocarbon receptor antagonist to a patient for short-term and/or long-term in vivo repopulation benefits.

摘要翻译： 本发明涉及用于扩增造血干细胞/祖细胞并为有需要的人类患者提供造血功能的方法，试剂盒和组合物。 一方面，本发明涉及包含Notch激动剂和芳基烃受体拮抗剂的试剂盒和组合物。 本文还提供了使用包含Notch激动剂和芳基烃受体拮抗剂的试剂盒和组合物扩增造血干细胞/祖细胞的方法。 使用所公开的试剂盒，组合物和方法扩增的造血干细胞/祖细胞包括人脐带血干细胞/祖细胞，胎盘脐带血干/祖细胞和外周血干细胞。 本发明还涉及将患有Notch激动剂和芳基烃受体拮抗剂的组合扩增的造血干细胞/祖细胞施用于患者以获得短期和/或长期的体内再灌注益处。