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公开(公告)号：US07348176B2
公开(公告)日：2008-03-25
申请号：US11057772
申请日：2005-02-14
申请人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
发明人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
IPC分类号： C12M3/00
摘要： The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g., liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.
摘要翻译： 本发明提供了用于培养细胞的模块化室，其中可以调节室的体积而不损害室的密封或不可靠性。 本发明基于这样的原理，即可以使用在多个压缩水平下在板之间形成流体密封的垫圈来调节形成在夹住可压缩垫圈的两个板和基本上不可压缩的止挡件之间的腔室的体积。 本发明使得能够在基本上平行的和刚性的板之间培养细胞，其中可以使用相对较大体积的细胞来种植细胞，并且减少用于灌注的保留体积，而不打开或以其他方式分解系统以损害其液体密度和不育。 因此可以将新的封闭的，模块化和可扩展的细胞培养室灌注并用于培养器官（例如肝）辅助系统中具有高水平细胞功能的细胞（例如肝细胞），用于细胞的产生，用于生产细胞 或衍生的产品，如蛋白质或病毒，或用于处理生物液体以除去毒素（如氨），添加细胞合成产物或两者的系统。
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公开(公告)号：US06855542B2
公开(公告)日：2005-02-15
申请号：US10325437
申请日：2002-12-19
申请人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
发明人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
摘要： The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g., liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.
摘要翻译： 本发明提供了用于培养细胞的模块化室，其中可以调节室的体积而不损害室的密封或不可靠性。 本发明基于这样的原理，即可以使用在多个压缩水平下在板之间形成流体密封的垫圈来调节形成在夹住可压缩垫圈的两个板和基本上不可压缩的止挡件之间的腔室的体积。 本发明使得能够在基本上平行的和刚性的板之间培养细胞，其中可以使用相对较大体积的细胞来种植细胞，并且减少用于灌注的保留体积，而不打开或以其他方式分解系统以损害其液体密度和不育。 因此可以将新的封闭的，模块化和可扩展的细胞培养室灌注并用于培养器官（例如肝）辅助系统中具有高水平细胞功能的细胞（例如肝细胞），用于细胞的产生，用于生产细胞 或衍生的产品，如蛋白质或病毒，或用于处理生物液体以除去毒素（如氨），添加细胞合成产物或两者的系统。
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公开(公告)号：US20060003436A1
公开(公告)日：2006-01-05
申请号：US11057772
申请日：2005-02-14
申请人： Paul DiMilla , Maury Cosman , Rachel Halych , Lisa Romito , Chris Gemmiti , Kevin Odlum
发明人： Paul DiMilla , Maury Cosman , Rachel Halych , Lisa Romito , Chris Gemmiti , Kevin Odlum
IPC分类号： C12M3/00
摘要： The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g., liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.
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