-
公开(公告)号：US20240335776A1
公开(公告)日：2024-10-10
申请号：US18748880
申请日：2024-06-20
申请人： Repligen Corporation
发明人： Rudolf Pavlik , Ralf Kuriyel
CPC分类号： B01D35/26 , B01D29/11 , B01D29/66 , B01D29/902 , B01D29/908 , B01D65/02 , B01D69/08 , C12M29/16 , C12M41/44 , B01D2201/165 , B01D2321/04 , B01D2321/12
摘要： A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
-
公开(公告)号：US20240076594A1
公开(公告)日：2024-03-07
申请号：US18509131
申请日：2023-11-14
发明人： Barbara Paldus , Mark Selker
CPC分类号： C12M23/02 , C12M23/40 , C12M23/44 , C12M23/58 , C12M25/06 , C12M27/00 , C12M27/02 , C12M27/16 , C12M29/10 , C12M29/16 , C12M29/18 , C12M33/14 , C12M41/12 , C12M41/26 , C12M41/44
摘要： A method of carrying out a bioreaction in a multivessel bioreactor system may include flowing a culture medium at an initial flow rate through one or more fluidic paths between a master vessel and one or more slave vessels, detecting a first culture medium parameter value in the master vessel or the slave vessels, transmitting the first culture medium parameter value to a control system, determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value, and controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the one or more fluidic paths. The control system may adjust the flow rate of the culture medium to maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the one or more slave vessels.
-
公开(公告)号：US11913031B2
公开(公告)日：2024-02-27
申请号：US16905987
申请日：2020-06-19
发明人： Yaakov Nahmias
IPC分类号： C12N5/071 , C12M3/06 , C12M1/34 , C12N5/0797
CPC分类号： C12N5/0697 , C12M23/16 , C12M41/12 , C12M41/44 , C12N5/067 , C12N5/069 , C12N5/0623 , C12N5/0686 , C12N2500/30 , C12N2500/32 , C12N2501/30 , C12N2501/305
摘要： Provided are in-vitro methods and devices for sustaining a synchronized circadian rhythm in cells of a cell culture by exposing the cells to a continuous flow of medium and to at least two stimuli provided in an oscillating manner with a periodicity of 24±4 hours, wherein a first stimulus and a second stimulus of said at least two stimuli are distinct, wherein said first stimulus is provided in a first time period and reaches a first peak in a first peak time period, and wherein a second stimulus is provided in a second time period and reaches a second peak in a second peak time period, and wherein an interval between end of time period of said first peak and beginning of said time period of said second peak is at least about 2 hours.
-
公开(公告)号：US20230374441A1
公开(公告)日：2023-11-23
申请号：US18028217
申请日：2021-09-15
发明人： Xiaoliang GUO , Jialin YAO , Yuanfang SHANG
摘要： A method for automatic cell centrifugal cleaning and medium replacement includes: obtaining a cell volume and a critical value of a centrifugal device after centrifugal stabilization of the centrifugal device; and performing, according to a magnitude relationship between the cell volume and the critical value of the centrifugal device, a velocity reduction process on a centrifugal chamber by combining the cell volume and the centrifugal chamber information. The extracted volume is changed by velocity-reduced rotation, and the rotation at a reduced velocity is adjusted according to the magnitude relationship between the cell volume and the critical value of the centrifugal device. The optimal cleaning and medium replacement effect on cells of different volumes can be realized. A high capture rate and a high medium replacement rate are obtained and maximized from different volumes of cell samples in the fixed parameters of the centrifugal chamber.
-
5.发明授权公开(公告)号：US11781104B2
公开(公告)日：2023-10-10
申请号：US16795696
申请日：2020-02-20
发明人： Huijun Gao , Mingsi Tong , Xinghu Yu , Linqi Zhang
CPC分类号： C12M41/44 , A61M5/16831 , A61M5/3286 , C12M41/48 , C12N15/89 , G01F23/292 , A61M2005/1588
摘要： The present invention provides an ultra-microinjection detection and control device based on lensless imaging and a method thereof. A lensless optical liquid level sensor is used to measure a change of a liquid level in an injection needle. A microinjection control unit is used to track the change of the liquid level and correct an injection pressure of the injection pump. Transmitted light generated by a parallel light source passes through a transparent glass tube of the injection needle. Then the transmitted light passes through a light filtering film to reduce the intensity of the parallel light source to a photosensitivity range of a micro linear array image sensor chip. Finally, the transmitted light enters the micro linear array image sensor chip, so that the micro linear array image sensor chip measures the change of the liquid level in the injection needle.
-
公开(公告)号：US20230279331A1
公开(公告)日：2023-09-07
申请号：US18005777
申请日：2021-07-29
发明人： Akira HIGUCHI
摘要： A culture device includes a culture vessel that contains a culture solution for culturing cells, a gas supply device that supplies a gas to the culture vessel, and a humidification device that humidifies the gas flowing from the gas supply device to the culture vessel. The humidification device includes a hollow fiber membrane filter that includes a hollow fiber membrane through which the gas from the gas supply device passes and a casing that accommodates the hollow fiber membrane, a water supply device that fills the casing of the hollow fiber membrane filter with water, and a first heater that heats the hollow fiber membrane filter.
-
公开(公告)号：US20230272328A1
公开(公告)日：2023-08-31
申请号：US18175144
申请日：2023-02-27
发明人： Justin Palmer , Scott Cambron , James B. hOYING , Michael Golway
摘要： The present disclosure relates to a bioreactor assembly having a housing defining an interior chamber; a lid assembly removably coupled to the housing and enclosing the interior chamber; and a gimbal assembly disposable within the interior chamber. The gimbal assembly includes a cradle configured to hold an organ or organ scaffold and an arm assembly configured to move the cradle between a plurality of positions. The bioreactor assembly also includes an ultrasound imaging unit positioned to capture volumetric and/or spatial data of the organ or organ scaffold.
-
公开(公告)号：US20230242858A1
公开(公告)日：2023-08-03
申请号：US18162463
申请日：2023-01-31
摘要： There is provided a cell detaching apparatus for detaching cells from a base material by applying an ultrasonic vibration via an acoustic matching liquid, with reduced variations of the vibration transmitted to the cells during application of the ultrasonic vibration. A cell detaching apparatus for detaching cells placed on a base material floating on an acoustic matching liquid, from the base material includes a holding unit configured to hold the acoustic matching liquid, a detaching unit configured to detach the cells from the base material by applying an ultrasonic vibration to the cells through the acoustic matching liquid, and a control unit configured to perform control to maintain a constant height of a fluid level of the acoustic matching liquid held by the holding unit with respect to the base material.
-
公开(公告)号：US20230212500A1
公开(公告)日：2023-07-06
申请号：US17976331
申请日：2022-10-28
摘要： Embodiments of the provided technology relate to a gas flow and liquid flow regulation system within a cell culture instrument. Embodiments of the gas and liquid flow regulation system include a pressurizable gas-mixing chamber, a cell culture compartment that includes a cell culture vessel having and a gas space, and a gas flow system. The gas flow system is driven by gas pressure, and is adaptable to provide an atmospheric condition of hypoxia and hyperbaric pressure within the cell culture compartment. The liquid flow regulation system is driven by hydraulic pressure.
-
公开(公告)号：US11686726B2
公开(公告)日：2023-06-27
申请号：US16470607
申请日：2017-12-27
发明人： Ola Lind , Johan Fredrik Avallin , Andreas Torbjorn Lundin , Nils Norrman , Ronnie Palmgren , Gustav Jose Rodrigo
IPC分类号： G01N33/543 , C12M3/00 , C12M3/06 , C12M1/00 , C12M1/34 , C12N13/00 , B01D21/00 , B03C5/00 , B03C5/02 , B03C1/30 , B03C1/28 , B03C1/01
CPC分类号： G01N33/54326 , B01D21/0009 , B03C1/30 , B03C5/00 , B03C5/02 , C12M23/48 , C12M27/16 , C12M29/00 , C12M41/44 , C12M45/07 , C12M47/04 , C12M47/10 , C12N13/00 , G01N33/5436 , B03C1/01 , B03C1/288 , B03C2201/26 , G01N2333/31
摘要： A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.
-
-
-
-
-
-
-
-
-
搜索结果
303 条记录 (耗时 0.024 秒)
