公开(公告)号：US06790659B2

公开(公告)日：2004-09-14

申请号：US10109543

申请日：2002-03-28

申请人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

发明人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

IPC分类号： C12N500

CPC分类号： C12N5/0601 ,  C12N2500/82

摘要： The present invention provides a process for large scale in vitro production of amoebocytes of Indian Horseshoe Crab (Tachypleus gigas) (T. gigas) from dissected gill flaps of T. gigas, in Leibovitz L-15 culture medium concentration (2×), to provide enhanced generation of amoebocytes. The process comprises the steps of: dissecting gill flaps of T. gigas; washing the gill flaps with an antibiotic solution followed by alcohol; culturing the gill flaps in tissue culture plates of sterile saline on a Rocker platform; culturing further the gill flaps in Leibovitz L-15 culture medium (2×); purging the gill flaps with Tween 80 solution; and purging again the gill flaps with horseshoe crab serum, while keeping the gill flaps in the culture medium viable for 90 days by feeding with fresh medium at an interval of 10-15 days to enable the enhanced release of amoebocytes both within and outside the gill flaps.

摘要翻译： 本发明提供了一种在Leibovitz L-15培养基浓度（2x）中大规模体外生产印度马蹄蟹（Tachypleus gigas）（Tigigus gigas）（T.gigas））来自解剖的T.gigas鳃片的变形细胞的方法，以提供 增强的变形细胞生成。 该过程包括以下步骤：解剖T.gigas的鳃片; 用抗生素溶液洗涤鳃瓣，然后用酒精洗涤; 在Rocker平台上的无菌盐水组织培养板中培养鳃瓣; 在Leibovitz L-15培养基（2x）中进一步培养鳃瓣; 用吐温80溶液清洗鳃瓣; 并用马蹄蟹血清再次清除鳃瓣，同时通过以10-15天的间隔喂养新鲜培养基将鳃瓣保持在培养基中90天，以使得能够在鳃内和外部增强释放的变形细胞 襟翼

公开(公告)号：US07074433B2

公开(公告)日：2006-07-11

申请号：US10112078

申请日：2002-03-29

申请人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

发明人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

IPC分类号： A61K35/56

CPC分类号： A61K35/618

摘要： The present invention relates to a process for the cure and control of Diabetes mellitus using natural products isolated from Perna viridis.

摘要翻译： 本发明涉及一种使用从白蛉分离的天然产物治疗和控制糖尿病的方法。