公开(公告)号：US5215882A

公开(公告)日：1993-06-01

申请号：US444031

申请日：1989-11-30

申请人： Chander Bahl ,  Rhonda Lang ,  Leopoldo G. Mendoza

发明人： Chander Bahl ,  Rhonda Lang ,  Leopoldo G. Mendoza

IPC分类号： C07H21/00 ,  C12Q1/68 ,  G01N33/543

CPC分类号： C07H21/00 ,  C12Q1/6834 ,  G01N33/54353 ,  Y10S435/81 ,  Y10S436/808

摘要： Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.