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公开(公告)号：US5858724A
公开(公告)日：1999-01-12
申请号：US683007
申请日：1996-07-16
IPC分类号： C07K14/745 , C12N15/12 , C07K14/245 , C12N5/10
CPC分类号： C07K14/745
摘要： Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.
摘要翻译： 重组兔组织因子在细菌宿主中克隆并产生。 该蛋白质相对不溶并具有几个二硫键，需要进行特殊的修饰以便在商业水平上表达和纯化。 通过将组织因子表达为具有细菌酶的融合蛋白，硫氧还蛋白的溶解度增加。 使用硫氧还蛋白还原酶缺陷宿主有助于生物活性的适当三级结构。
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公开(公告)号：US06589783B2
公开(公告)日：2003-07-08
申请号：US09548880
申请日：2000-04-13
申请人： Robert E. Novy , Scott A. Monsma
发明人： Robert E. Novy , Scott A. Monsma
IPC分类号： C12N1500
CPC分类号： C12N15/86 , C12N15/70 , C12N2710/14143
摘要： A triple host vector is described which is capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vector includes three promoters, one each for vertebrate, bacterial and insect host cells, so that the protein coding sequence can be cloned into the vector once and the vector can be used to express protein in all three types of host cells. The arrangement and selection of the promoters contributes to expression characteristics in all three host systems that is comparable to single host expression vectors.
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公开(公告)号：US5756302A
公开(公告)日：1998-05-26
申请号：US683060
申请日：1996-07-16
IPC分类号： C07K16/08 , G01N33/569 , C12Q1/70 , C07K4/02 , G01N33/53
CPC分类号： C07K16/085 , G01N33/56994
摘要： An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of LDGYPSDYDPFVENAQIY (SEQ ID NO:1) and LDGYPSDYDPFEENAQIY (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.
摘要翻译： 针对具有选自LDGYPSDYDPFVENAQIY（SEQ ID NO：1）和LDGYPSDYDPFEENAQIY（SEQ ID NO：2）的氨基酸序列的肽的抗原产生的抗体有利地用于间接免疫测定中以检测活性物质的存在 人类疱疹病毒-6患者样本。
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4.发明授权Antibody to carboxy-terminus of human herpesvirus 6 immediate early protein 失效人类疱疹病毒6的早期蛋白质的羧基末端的抗体
公开(公告)号：US6103878A
公开(公告)日：2000-08-15
申请号：US84574
申请日：1998-05-26
IPC分类号： C07K16/08 , G01N33/569 , A61K39/42 , C07K14/03
CPC分类号： C07K16/085 , G01N33/56994
摘要： An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Val Glu Asn Ala Gln Ile Tyr (SEQ ID NO:1) and Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Glu Glu Asn Ala Gln Ile Tyr (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.
摘要翻译： 针对具有选自Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Val Glu Asn Ala Gln Ile Tyr（SEQ ID NO：1）和Leu Asp Gly Tyr的氨基酸序列的肽表位产生的抗体 Pro Ser Asp Tyr Asp Pro Phe Glu Glu Asn Ala Gln Ile Tyr（SEQ ID NO：2）有利地用于间接免疫测定以检测患者样品中活性人疱疹病毒-6的存在。
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公开(公告)号：US5856144A
公开(公告)日：1999-01-05
申请号：US878379
申请日：1997-06-18
CPC分类号： C12N15/66
摘要： A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.
摘要翻译： 用于直接克隆PCR方法产物的载体在线性化DNA片段的一端或两端掺入单核苷酸突出端。 根据需要，单核苷酸突出端是尿嘧啶或肌苷残基，以促进所需PCR产物的克隆。
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