公开(公告)号：US20080248468A1

公开(公告)日：2008-10-09

申请号：US11792902

申请日：2005-12-13

申请人： Manfred Kubbies ,  Robert Macek ,  Carola Ries

发明人： Manfred Kubbies ,  Robert Macek ,  Carola Ries

IPC分类号： C12Q1/68 ,  C12N15/87 ,  C12N15/63 ,  C12N5/06 ,  C07K16/00

CPC分类号： C12N15/111 ,  C12N2310/111 ,  C12N2310/14

摘要： The present invention is directed to a method for inactivation of expression of a gene in a eucaryotic cell comprising (i) transfection of a eucaryotic cell with DNA comprising an expression cassette for expression of a cell surface protein and an expression cassette for expression of a RNAi compound, said compound being capable of inactivating expression of said gene, wherein said expression cassette for expression of a cell surface protein and said expression cassette for expression of a RNAi compound are located on the same vector DNA, and (ii) enrichment and/or selection of cells which express said cell surface protein.

摘要翻译： 本发明涉及一种用于灭活真核细胞中基因表达的方法，包括（i）用包含用于表达细胞表面蛋白的表达盒和用于表达RNAi的表达盒的DNA转染真核细胞 化合物，所述化合物能够使所述基因的表达失活，其中用于表达细胞表面蛋白的表达盒和所述表达RNAi化合物的表达盒位于相同的载体DNA上，和（ii）富集和/或 选择表达所述细胞表面蛋白的细胞。