DETECTION OF E. COLI STRAINS TY2482 AND LB226692
    2.
    发明申请
    DETECTION OF E. COLI STRAINS TY2482 AND LB226692 审中-公开
    大肠杆菌菌株TY2482和LB226692的检测

    公开(公告)号:US20120315632A1

    公开(公告)日:2012-12-13

    申请号:US13492369

    申请日:2012-06-08

    IPC分类号: C12Q1/68 C12M1/34 C40B20/00

    CPC分类号: C12Q1/689 C12Q2600/156

    摘要: The present invention relates generally to strain typing of Escherichia coli TY2482 and/or LB226692, and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. In particular embodiments, a genetic detection assay (e.g., SNP detection assay, sequencing, etc.) is used to identify mutations in the aggregative adherence fimbria I gene cluster fimbrial subunit (aggA) gene to identify the TY2482 strain, including mutations at position 103, 158, 188, 191, 197, and 271.

    摘要翻译: 本发明一般涉及大肠杆菌TY2482和/或LB226692的菌株分型,并且提供了当例如与分子量或碱组成分析组合时可用于此目的的方法,组合物和试剂盒。 在特定实施方案中,遗传检测测定(例如,SNP检测测定,测序等)用于鉴定聚集性粘附性fimbria I基因簇毛细胞亚基(aggA)基因中的突变以鉴定TY2482菌株,包括位置103的突变 ,158,188,191,197和271。