公开(公告)号：US5952654A

公开(公告)日：1999-09-14

申请号：US960305

申请日：1997-10-29

申请人： Roger Giese

发明人： Roger Giese

IPC分类号： G01N27/62 ,  H01J49/04 ,  H01J49/16

CPC分类号： H01J49/0418 ,  H01J49/0409 ,  Y10T436/24

摘要： Methods of releasing and analyzing substrates such as DNA, comprising: a) covalently or ligandly binding substrate to a first electrode via a release group, which release group is cleavable in response to applied energy; b) introducing an electrical field so as to establish a charge potential between the first electrode and a second electrode separated by a vacuum or gas phase from the first electrode, the strength of such field sufficient to bristle said covalently-bound or ligandly-bound substrate; and c) applying sufficient energy directly to the release group to cleave the release group and release the substrate into a vacuum or gas phased

摘要翻译： 释放和分析诸如DNA的底物的方法包括：a）通过释放基团将底物共价或配体结合到第一电极，该释放基团可响应于施加的能量而可切割; b）引入电场，以便在第一电极和由第一电极分离的真空或气相的第二电极之间建立充电电势，足以使所述共价结合或配体结合的底物刷毛的这种场强度 ; 和c）将足够的能量直接施加到释放基团以切割释放组并将基底释放到真空或气相中

公开(公告)号：US20180321226A1

公开(公告)日：2018-11-08

申请号：US16021941

申请日：2018-06-28

申请人： Roger GIESE ,  Poguang WANG

发明人： Roger GIESE ,  Poguang WANG

IPC分类号： G01N33/52 ,  C07C217/48 ,  C07C217/58

CPC分类号： G01N33/52 ,  C07C217/48 ,  C07C217/58 ,  G01N2458/15 ,  G01N2560/00

摘要： The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.

公开(公告)号：US10161933B2

公开(公告)日：2018-12-25

申请号：US16021941

申请日：2018-06-28

申请人： Roger Giese ,  Poguang Wang

发明人： Roger Giese ,  Poguang Wang

IPC分类号： G01N33/52 ,  G01N24/00 ,  C07C217/58 ,  C07C217/48

摘要： The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.