公开(公告)号：US5830720A

公开(公告)日：1998-11-03

申请号：US386198

申请日：1995-02-09

申请人： August Bock ,  Robert Gary Sawers ,  Michael Jarsch ,  Roland Herbst

发明人： August Bock ,  Robert Gary Sawers ,  Michael Jarsch ,  Roland Herbst

IPC分类号： C12N1/21 ,  C12N15/70 ,  C12N15/63 ,  C07H21/04 ,  C12P21/02

CPC分类号： C12N15/70

摘要： The recombinant DNA according to the present invention contains a regulator region which is different from the pfl gene; a promoter region upstream from the gene which contains a -35/-10 promoter sequence; a regulator region upstream from the promoter region which contains a sequence (I) 5'-GAGATATGATCTATATCAATTTC-3' or a 23 base pair sequence which is at least 80% identical to positions 6-10 and 15-19 of said sequence (I) but not having C at position 8 and/or G at position 17. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule and methods are also described for expression the DNA.

摘要翻译： 根据本发明的重组DNA含有与pfl基因不同的调节区; 该基因上游的启动子区含有-35 / -10启动子序列; 包含序列（I）5'-GAGATATGATCTATATCAATTTC-3'或与所述序列（I）的6-10和15-19位置至少80％相同的23个碱基对序列的启动子区域上游的调节区） 但在位置8处不具有C和/或在位置17处具有G。根据本发明的表达载体含有连接到合适的载体分子中的本发明的重组DNA，还描述了用于表达DNA的方法。