Recombinant DNA and expression vector for the repressible and inducible
expression of foreign genes
    1.
    发明授权
    Recombinant DNA and expression vector for the repressible and inducible expression of foreign genes 失效
    重组DNA和表达载体,用于外源基因的抑制和诱导表达

    公开(公告)号:US5830720A

    公开(公告)日:1998-11-03

    申请号:US386198

    申请日:1995-02-09

    CPC分类号: C12N15/70

    摘要: The recombinant DNA according to the present invention contains a regulator region which is different from the pfl gene; a promoter region upstream from the gene which contains a -35/-10 promoter sequence; a regulator region upstream from the promoter region which contains a sequence (I) 5'-GAGATATGATCTATATCAATTTC-3' or a 23 base pair sequence which is at least 80% identical to positions 6-10 and 15-19 of said sequence (I) but not having C at position 8 and/or G at position 17. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule and methods are also described for expression the DNA.

    摘要翻译: 根据本发明的重组DNA含有与pfl基因不同的调节区; 该基因上游的启动子区含有-35 / -10启动子序列; 包含序列(I)5'-GAGATATGATCTATATCAATTTC-3'或与所述序列(I)的6-10和15-19位置至少80%相同的23个碱基对序列的启动子区域上游的调节区) 但在位置8处不具有C和/或在位置17处具有G。根据本发明的表达载体含有连接到合适的载体分子中的本发明的重组DNA,还描述了用于表达DNA的方法。

    Cholesterol oxidase from Brevibacterium sterolicum
    4.
    发明授权
    Cholesterol oxidase from Brevibacterium sterolicum 失效
    来自短杆菌的胆固醇氧化酶

    公开(公告)号:US5916759A

    公开(公告)日:1999-06-29

    申请号:US532795

    申请日:1996-01-05

    申请人: Michael Jarsch

    发明人: Michael Jarsch

    CPC分类号: C12N9/0006 C07K2319/00

    摘要: The invention concerns a cholesterol oxidase, a process for the production of a recombinant cholesterol oxidase, a DNA sequence suitable for this process which causes a cytoplasmatic expression of the recombinant cholesterol oxidase in a host bacterium as well as the recombinant cholesterol oxidase obtianed in this way.

    摘要翻译: PCT No.PCT / EP94 / 01394 Sec。 371日期1996年1月5日 102(e)日期1996年1月5日PCT提交1994年5月2日PCT公布。 公开号WO94 / 25603 日期1994年11月10日本发明涉及一种胆固醇氧化酶,一种生产重组胆固醇氧化酶的方法,适用于该过程的DNA序列,其引起宿主细菌中重组胆固醇氧化酶的细胞质表达以及重组胆固醇 氧化酶以这种方式恶化。

    Expression vector for the regulatable expression of foreign genes in prokaryotes
    5.
    发明授权
    Expression vector for the regulatable expression of foreign genes in prokaryotes 失效
    外源基因在原核生物中可调节表达的表达载体

    公开(公告)号:US06190906B1

    公开(公告)日:2001-02-20

    申请号:US07300357

    申请日:1989-01-23

    IPC分类号: C12N1563

    CPC分类号: C12N15/63

    摘要: The invention provides an expression vector useful in expressing foreign genes in procaryotes. The vector consists of the promotor/operator region and initiation point of translation of the mg1 operon. This combination is referred to as a regulation sequence. In order to prepare this, desired portions of the mg1 operon are cut from the genome of a cell which can utilize galactose via appropriate restriction endonucleases. This is then inserted into a DNA vector.

    摘要翻译: 本发明提供了可用于在原核生物中表达外源基因的表达载体。 载体由启动子/操纵子区和mg1操纵子的翻译起始点组成。 这种组合被称为调节序列。 为了制备此,从可通过合适的限制性内切核酸酶利用半乳糖的细胞的基因组切割出mg1操纵子的所需部分。 然后将其插入DNA载体。