公开(公告)号：US20090214513A1

公开(公告)日：2009-08-27

申请号：US11911908

申请日：2006-04-20

申请人： Xiaotian Zhong ,  Ronald William Kriz ,  Mark Lloyd Stahl

发明人： Xiaotian Zhong ,  Ronald William Kriz ,  Mark Lloyd Stahl

IPC分类号： A61K38/46 ,  C12N5/06 ,  C12N15/87 ,  C12N5/08 ,  C12P21/00 ,  A61K38/02

CPC分类号： C12P21/02

摘要： The present invention features mammalian expression systems with improved production yields, and method of using these systems to produce desired proteins. In one embodiment, the expression systems of the present invention comprise genetically-engineered mammalian host cells cultured in a medium that contains an effective amount of heparin or heparin-like molecules. The presence of heparin or heparin-like molecules significantly increases protein production by the cultured cells. The present invention also features the use of constitutively-active components of FGFR-I-mediated signal transduction pathways to improve protein production by cultured mammalian cells. Co-expression of such a component with a protein of interest markedly increases the production yield of the protein of interest.

摘要翻译： 本发明具有提高生产产量的哺乳动物表达系统以及使用这些系统产生所需蛋白质的方法。 在一个实施方案中，本发明的表达系统包含在含有有效量的肝素或类肝素分子的培养基中培养的遗传工程化的哺乳动物宿主细胞。 肝素或肝素样分子的存在显着增加培养细胞的蛋白质产生。 本发明还特征在于使用FGFR-1介导的信号转导途径的组成型活性成分来改善培养的哺乳动物细胞的蛋白质产生。 这种与感兴趣的蛋白质的组分的共表达显着增加了目的蛋白质的产量。