公开(公告)号：US20100248207A1

公开(公告)日：2010-09-30

申请号：US12665381

申请日：2009-06-19

申请人： Ryan Raz ,  Anthony Presta ,  Laurent Safar ,  Frédéric Revah

发明人： Ryan Raz ,  Anthony Presta ,  Laurent Safar ,  Frédéric Revah

IPC分类号： C12N5/00 ,  C12M1/00

CPC分类号： G01N1/4077 ,  B01D61/147 ,  G01N2001/4088 ,  G01N2203/0089 ,  G01N2203/0094

摘要： The present invention relates to a method for the separation of biological objects in a solution which have different viscoelastic properties, wherein said method comprises a filtration step allowing the higher viscoelastic biological objects to pass through the membrane while retaining the lower viscoelastic biological objects above the membrane, and a recovery step wherein the separated lower viscoelastic biological objects are recovered above or onto the membrane and/or the separated higher viscoelastic biological objects are recovered in the filtrate. Advantageously, the biological objects are cells. More advantageously, the recovered cells are viable cells. In one preferred embodiment, the cells are tumor cells. In another preferred embodiment, the cells are fetal cells and the method finds an application in prenatal diagnosis.

摘要翻译： 本发明涉及一种用于分离具有不同粘弹性特性的溶液中的生物物质的方法，其中所述方法包括使较高粘弹性生物体通过膜同时将低粘弹性生物体保留在膜上方的过滤步骤 以及回收步骤，其中将分离的下部粘弹性生物体在膜上方或其上回收和/或分离的较高粘弹性生物体在滤液中回收。 有利地，生物体是细胞。 更有利地，回收的细胞是活细胞。 在一个优选的实施方案中，细胞是肿瘤细胞。 在另一个优选的实施方案中，细胞是胎儿细胞，并且该方法在产前诊断中找到应用。

公开(公告)号：US06210331B1

公开(公告)日：2001-04-03

申请号：US09219323

申请日：1998-12-23

申请人： Ryan Raz

发明人： Ryan Raz

IPC分类号： A61B800

CPC分类号： G01S15/8925 ,  G01S15/8936 ,  G01S15/8965 ,  G10K11/30

摘要： An ultrasonic imaging system incorporates an imaging device utilizing confocal acoustic focusing of ultrasonic transducers together with apparatus for moving points of focus of the transducers to scan a volume to be imaged and gather data as to the acoustic reflectivity of matter within the volume, the gathered data being used to construct an image of the volume. In a preferred arrangement the point of focus of each transducer is moved in three dimensions. Use of confocal techniques can permit enhanced resolution to be obtained compared with conventional ultrasonic imaging techniques. In a preferred arrangement, the confocal focusing system associated with each transducer includes a pinhole which is bridged by an acoustically transparent but optically reflective diaphragm, and acoustic reflectivity data is recovered by interrogating each diaphragm by means of an optical interferometer. Optical signals from the interferometer may be scanned over an array of optical sensors such as a charge coupled device array to segment and temporarily store the optical data.

摘要翻译： 超声成像系统包括利用超声波换能器的共聚焦声学聚焦的成像装置和用于移动换能器的焦点的扫描要成像的体积的装置的装置，并收集关于体积内的物质的声反射率的数据，所收集的数据 用于构建卷的图像。 在优选的布置中，每个换能器的焦点在三个维度上移动。 与传统的超声成像技术相比，使用共聚焦技术可以获得更高的分辨率。 在优选的布置中，与每个换能器相关联的共聚焦点系统包括由声透明但光反射的光阑桥接的针孔，并且借助于光学干涉仪询问每个隔膜来恢复声学反射率数据。 可以通过诸如电荷耦合器件阵列的光学传感器阵列来扫描来自干涉仪的光学信号，以分段和临时存储光学数据。