公开(公告)号：US07763437B1

公开(公告)日：2010-07-27

申请号：US11482277

申请日：2006-07-07

申请人： Marc G. Caron ,  Martin Beaulieu ,  Raul R. Gainetdinov ,  Tatiana D. Sotnikova ,  Sébastien Marion

发明人： Marc G. Caron ,  Martin Beaulieu ,  Raul R. Gainetdinov ,  Tatiana D. Sotnikova ,  Sébastien Marion

IPC分类号： G01N33/53

CPC分类号： G01N33/74 ,  G01N2333/726

摘要： A method of screening a candidate compound for βArrestin mediated anti-G protein coupled receptor signaling activity is comprises: (a) contacting said candidate compound to a βArrestin signaling complex or a constituent thereof, under conditions in which a signaling complex is formed; and then (b) detecting the presence or absence of disruption of said signaling complex, disruption of said complex indicating said compound has βArrestin mediated anti-G protein coupled receptor signaling activity. Compositions and kits for carrying out the method are also described.

摘要翻译： 一种筛选候选化合物以控制Arrestin介导的抗G蛋白偶联受体信号传导活性的方法包括：（a）在信号传导复合体是在其下的条件下将所述候选化合物与一种Arrestin信号传导复合物或其组分接触 形成 然后（b）检测所述信号复合物的破坏的存在或不存在，指示所述化合物的所述复合物的破坏具有Arrestin介导的抗G蛋白偶联受体信号传导活性。 还描述了用于实施该方法的组合物和试剂盒。

公开(公告)号：US07999075B2

公开(公告)日：2011-08-16

申请号：US12816764

申请日：2010-06-16

申请人： Marc G. Caron ,  Martin Beaulieu ,  Raul R. Gainetdinov ,  Tatiana D. Sotnikova ,  Sébastien Marion

发明人： Marc G. Caron ,  Martin Beaulieu ,  Raul R. Gainetdinov ,  Tatiana D. Sotnikova ,  Sébastien Marion

IPC分类号： C12N11/18 ,  C12N9/12 ,  C12N9/14 ,  C07K14/47

CPC分类号： G01N33/74 ,  G01N2333/726

摘要： A method of screening a candidate compound for βArrestin mediated anti-G protein coupled receptor signaling activity is comprises: (a) contacting said candidate compound to a βArrestin signaling complex or a constituent thereof, under conditions in which a signaling complex is formed; and then (b) detecting the presence or absence of disruption of said signaling complex, disruption of said complex indicating said compound has βArrestin mediated anti-G protein coupled receptor signaling activity. Compositions and kits for carrying out the method are also described.

摘要翻译： 一种筛选候选化合物以控制Arrestin介导的抗G蛋白偶联受体信号传导活性的方法包括：（a）在信号传导复合体是在其下的条件下将所述候选化合物与一种Arrestin信号传导复合物或其组分接触 形成 然后（b）检测所述信号复合物的破坏的存在或不存在，指示所述化合物的所述复合物的破坏具有Arrestin介导的抗G蛋白偶联受体信号传导活性。 还描述了用于实施该方法的组合物和试剂盒。