公开(公告)号：US07358067B2

公开(公告)日：2008-04-15

申请号：US09982531

申请日：2001-10-19

申请人： Astrid Vrang ,  Søren Michael Madsen ,  Lars Bredmose ,  Peter Ravn ,  Jose Arnau ,  Mads Gronvald Johnsen ,  Anne Cathrine Steenberg ,  Hans Israelsen

发明人： Astrid Vrang ,  Søren Michael Madsen ,  Lars Bredmose ,  Peter Ravn ,  Jose Arnau ,  Mads Gronvald Johnsen ,  Anne Cathrine Steenberg ,  Hans Israelsen

IPC分类号： C12P21/00 ,  C12N1/21

CPC分类号： C12N15/74 ,  C12N1/00 ,  C12N9/22 ,  C12N15/67

摘要： The invention provides a fed-batch or continuous process for producing heterologous peptides, polypeptides or proteins in lactic acid bacteria, comprising the steps of (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein, and appropriate regulatory nucleotide sequences such as regulatable promoters and signal peptides to control the expression of the coding sequence and the secretion of gene product, (ii) cultivating the recombinant bacterium under or continuous cultivation conditions to express the gene, and (iii) harvesting the recombinant bacterium or the gene product. Preferably, the cultivation medium is a chemically defined or synthetic medium optionally supplemented with yeast extract.

摘要翻译： 本发明提供了用于在乳酸菌中产生异源肽，多肽或蛋白质的补料分批或连续方法，包括以下步骤：（i）构建重组乳酸菌，其包含编码异源肽，多肽或蛋白质的核苷酸序列， 以及适当的调控核苷酸序列如可调节的启动子和信号肽，以控制编码序列的表达和基因产物的分泌，（ii）在培养条件下或连续培养条件下培养重组细菌以表达基因，和（iii）收获 重组细菌或基因产物。 优选地，培养基是任选地补充有酵母提取物的化学上定义的或合成的培养基。