公开(公告)号：US12122995B2

公开(公告)日：2024-10-22

申请号：US16726163

申请日：2019-12-23

申请人： DNA2.0, INC.

发明人： Jeremy Minshull ,  Mark Welch ,  Sridhar Govindrajan ,  Maggie Lee ,  Kate Caves ,  Jon Ness

IPC分类号： C12N9/12 ,  C12N1/16 ,  C12N1/18 ,  C12N15/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/63 ,  C12N15/67 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C12R1/84 ,  C12R1/865

CPC分类号： C12N1/165 ,  C12N1/185 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/1082 ,  C12N15/52 ,  C12N15/625 ,  C12N15/63 ,  C12N15/81 ,  C12N15/815 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/90 ,  C12Y207/00 ,  C12Y207/07 ,  C07K2319/09 ,  C12N15/67 ,  C12N2800/90 ,  C12N2830/007 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2840/203 ,  C12R2001/84 ,  C12R2001/865

摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

公开(公告)号：US12121531B2

公开(公告)日：2024-10-22

申请号：US17519120

申请日：2021-11-04

申请人： Flagship Pioneering Innovations VI, LLC

发明人： Theonie Anastassiadis ,  David Charles Donnell Butler ,  Neil Kubica ,  Qingyi Li

IPC分类号： A61K31/7088 ,  A61K31/712 ,  A61K31/7125 ,  C12N15/11 ,  C12N15/67

CPC分类号： A61K31/7088 ,  A61K31/712 ,  A61K31/7125 ,  C12N15/11 ,  C12N15/67 ,  C12N2310/313 ,  C12N2310/321 ,  C12N2310/322

摘要： The invention relates generally to tRNA-based effector molecules having a non-naturally occurring modification and methods relating thereto.

公开(公告)号：US20240344062A1

公开(公告)日：2024-10-17

申请号：US18682754

申请日：2022-08-10

申请人： THE BROAD INSTITUTE, INC.

发明人： Ahmed H. Badran ,  Fan Liu ,  Sinisa Bratulic

IPC分类号： C12N15/11 ,  C12N15/10 ,  C12N15/67 ,  C12N15/70

CPC分类号： C12N15/11 ,  C12N15/1058 ,  C12N15/67 ,  C12N15/70

摘要： The present disclosure relates to compositions, methods and kits for enhancing ribosomal activities in a host cell, especially improvement of the translation activity of heterologous ribosomes within a host cell. Specifically, the instant disclosure provides a number of evolved rRNA sequences, which were remarkably identified to possess enhanced translation activities, improved orthogonal-ribosome binding site (o-RBS) and orthogonal anti-ribosome binding site (o-antiRBS) sequences, host cells possessing deletion or disruption of ribosome hibernation promoting factor (HPF) that thereby exhibit enhanced propagation of selection phage constructs during (PACE), among other aspects. New transgenic organisms harboring heterologous ribosomes and operons are also provided.

公开(公告)号：US12104153B2

公开(公告)日：2024-10-01

申请号：US17552804

申请日：2021-12-16

申请人： Hoffmann-La Roche Inc.

发明人： Ravi Jagasia ,  Lars Jonson ,  Soren Rasmussen ,  Jacob Ravn ,  Disa Tehler ,  Dorthe Vang ,  Jesper Worm

IPC分类号： C12N15/113 ,  C12N15/67

CPC分类号： C12N15/113 ,  C12N15/67 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/346

摘要： The present invention relates to oligonucleotides which alter the splicing pattern of progranulin in cells, and their use in the treatment of neurological disorders.

公开(公告)号：US20240301432A1

公开(公告)日：2024-09-12

申请号：US17797560

申请日：2021-02-05

申请人： XBRANE BIOPHARMA AB ,  CLONEOPT AB

发明人： Kiavash MIRZADEH ,  Daniel DALEY ,  Patrick SHILLING

IPC分类号： C12N15/62 ,  C12N15/67 ,  C12N15/70 ,  C12P21/02

CPC分类号： C12N15/625 ,  C12N15/67 ,  C12N15/70 ,  C12P21/02 ,  C07K2319/02

摘要： The present invention provides a simple and inexpensive system for regulating signal peptide performance by using a synthetically evolved nucleotide sequence. The invention further relates to an expression vector comprising the nucleotide sequence. Additionally, the present invention relates to host cell comprising the expression vector. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.

公开(公告)号：US12054723B2

公开(公告)日：2024-08-06

申请号：US16898176

申请日：2020-06-10

申请人： BioNTech SE ,  TRON—Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg—Universität Mainz gG

发明人： Florian Eberle ,  Ugur Sahin ,  Andreas Kuhn ,  Britta Vallazza ,  Mustafa Diken

IPC分类号： C12N5/00 ,  A61K39/00 ,  A61K48/00 ,  C12N15/67 ,  C12N15/68 ,  C12N15/70 ,  C12N15/85 ,  C12P19/34 ,  C12P21/00

CPC分类号： C12N15/68 ,  A61K39/00 ,  A61K48/0066 ,  C12N15/67 ,  C12N15/70 ,  C12N15/85 ,  C12P19/34 ,  C12P21/00 ,  A61K2039/53 ,  C12N2830/50

摘要： The present invention relates to nucleic acid molecules containing poly (dA:dT) regions which are stabilized in E.-coli, methods of propagating such nucleic acid molecules in E. coli, methods of obtaining RNA, peptides or proteins using such nucleic acid molecules and to RNA which is obtained from such nucleic acid molecules and its use. In particular, the poly (dA:dT) regions contain at least one disruption by a sequence not encoding a sequence solely composed of A residues.

公开(公告)号：US12031170B2

公开(公告)日：2024-07-09

申请号：US17744300

申请日：2022-05-13

申请人： DANMARKS TEKNISKE UNIVERSITET

发明人： Peter Rugbjerg ,  Kira Sarup-Lytzen ,  Morten Sommer

IPC分类号： C12P7/42 ,  C12N1/21 ,  C12N9/16 ,  C12N15/10 ,  C12N15/67 ,  C12N15/72 ,  C12P1/02 ,  C12P1/04 ,  C12P5/02 ,  C12P7/40 ,  C12P7/64 ,  C12P21/02

CPC分类号： C12P7/42 ,  C12N9/16 ,  C12N15/1086 ,  C12N15/67 ,  C12N15/72 ,  C12P1/02 ,  C12P1/04 ,  C12P5/026 ,  C12P7/40 ,  C12P7/64 ,  C12P21/02

摘要： The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite. The invention further encompasses: a method for producing the genetically modified micro-organism; a method for producing a cellular metabolite with the genetically modified micro-organism; and use of the genetically modified micro-organism for producing a cellular metabolite.

公开(公告)号：US20240216545A1

公开(公告)日：2024-07-04

申请号：US18557575

申请日：2022-04-28

申请人： GENEVANT SCIENCES GMBH

发明人： Owen DALY ,  James HEYES ,  Kieu LAM

IPC分类号： A61K48/00 ,  C12N15/11 ,  C12N15/67 ,  C12N15/88

CPC分类号： A61K48/0066 ,  A61K48/0041 ,  C12N15/11 ,  C12N15/67 ,  C12N15/88 ,  C12N2800/60

摘要： The present disclosure provides, among other things, polynucleotide constructs, compositions, and methods of treating a disease or disorder, including administering to a subject in need thereof a composition comprising a polynucleotide construct comprising a 5′ UTR, a mRNA encoding a protein of interest, and a 3′ UTR.

公开(公告)号：US12023371B2

公开(公告)日：2024-07-02

申请号：US17127435

申请日：2020-12-18

申请人： ModernaTX, Inc.

发明人： Tirtha Chakraborty ,  Stephane Bancel ,  Stephen G. Hoge ,  Atanu Roy ,  Antonin De Fougerolles ,  Noubar B. Afeyan

IPC分类号： A61K39/00 ,  C12N15/67

CPC分类号： A61K39/00 ,  C12N15/67

摘要： The invention relates to compositions and methods for the manufacture and optimization of modified mRNA molecules via optimization of their terminal architecture.

公开(公告)号：US20240200069A1

公开(公告)日：2024-06-20

申请号：US18403466

申请日：2024-01-03

申请人： Arizona Board of Regents on behalf of Arizona State University

发明人： Alexander Green ,  Yu Zhou

IPC分类号： C12N15/113 ,  C12N15/67 ,  H03K19/20

CPC分类号： C12N15/113 ,  C12N15/67 ,  H03K19/20 ,  C12N2310/122 ,  C12N2310/531

摘要： Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.