摘要:
The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要:
The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.
摘要:
The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.