公开(公告)号：US08536314B2

公开(公告)日：2013-09-17

申请号：US12783301

申请日：2010-05-19

申请人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

发明人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

IPC分类号： C12N15/01 ,  A61K38/00

CPC分类号： C07K14/21 ,  C07K14/32 ,  Y02A50/473

摘要： The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.

摘要翻译： 本发明提供荧光蛋白的变体，其在其用作报告蛋白和/或分析中的性质方面得到改进。 特别地，提供荧光蛋白的变体，其发荧光更亮，显示改善的量子产率和/或具有偏移的激发或发射光谱。 根据本发明的荧光蛋白质除了用不与FMN共价结合至少一个另外的点突变的氨基酸取代半胱氨酸之外，还包含其LOV结构域。

公开(公告)号：US20100304435A1

公开(公告)日：2010-12-02

申请号：US12783301

申请日：2010-05-19

申请人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

发明人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

IPC分类号： C07K14/00 ,  C07H21/04 ,  C12P21/00

CPC分类号： C07K14/21 ,  C07K14/32 ,  Y02A50/473

摘要： The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.

摘要翻译： 本发明提供荧光蛋白的变体，其在其用作报告蛋白和/或分析中的性质方面得到改进。 特别地，提供荧光蛋白的变体，其发荧光更亮，显示改善的量子产率和/或具有偏移的激发或发射光谱。 根据本发明的荧光蛋白质除了用不与FMN共价结合至少一个另外的点突变的氨基酸取代半胱氨酸之外，还包含其LOV结构域。