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    Expression Vector for an Improved Protein Secretion
    2.
    发明申请
    Expression Vector for an Improved Protein Secretion 有权
    用于改善蛋白质分泌的表达载体

    公开(公告)号：US20140356929A1

    公开(公告)日：2014-12-04

    申请号：US14122562

    申请日：2012-05-25

    申请人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    发明人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    IPC分类号： C12N9/50

    CPC分类号： C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

    摘要： The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

    摘要翻译： 本发明的目的是改善蛋白质从宿主细胞的分泌，以增加发酵过程中蛋白质的产物产率。 这通过包含a）启动子序列和b）编码蛋白质的核酸序列的表达载体来实现。 该蛋白质包含信号肽和另外的氨基酸序列，并且信号肽包含与SEQ ID NO 2中指定的氨基酸序列至少80％相同的氨基酸序列，与氨基酸至少80％相同 SEQ ID NO 4中指定的序列与SEQ ID NO 6中指定的氨基酸序列至少80％相同，或者信号肽包含与至少一个所述序列结构同源的氨基酸序列。

    Fluorescent proteins, their production and use
    3.
    发明授权
    Fluorescent proteins, their production and use 失效
    荧光蛋白，其生产和使用

    公开(公告)号：US08536314B2

    公开(公告)日：2013-09-17

    申请号：US12783301

    申请日：2010-05-19

    申请人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

    发明人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

    IPC分类号： C12N15/01 ,  A61K38/00

    CPC分类号： C07K14/21 ,  C07K14/32 ,  Y02A50/473

    摘要： The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.

    摘要翻译： 本发明提供荧光蛋白的变体，其在其用作报告蛋白和/或分析中的性质方面得到改进。 特别地，提供荧光蛋白的变体，其发荧光更亮，显示改善的量子产率和/或具有偏移的激发或发射光谱。 根据本发明的荧光蛋白质除了用不与FMN共价结合至少一个另外的点突变的氨基酸取代半胱氨酸之外，还包含其LOV结构域。

    Esterases for separating plastics
    4.
    发明授权
    Esterases for separating plastics 失效
    用于分离塑料的酯酶

    公开(公告)号：US08580549B2

    公开(公告)日：2013-11-12

    申请号：US11989927

    申请日：2006-08-03

    申请人： Andreas Michels ,  André Pütz ,  Karl-Heinz Maurer ,  Thorsten Eggert ,  Karl-Erich Jäger

    发明人： Andreas Michels ,  André Pütz ,  Karl-Heinz Maurer ,  Thorsten Eggert ,  Karl-Erich Jäger

    IPC分类号： C12N9/18 ,  C12N9/16 ,  C12Q1/44 ,  C07K14/00 ,  C12P21/00

    CPC分类号： C12N9/18

    摘要： The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof.

    摘要翻译： 本发明涉及含有酯酶的试剂，以及其用于修整纤维的用途，特别是人造纤维，包含酯酶的洗涤和清洁剂以及相应的洗涤和清洁方法，以及额外的技术应用领域。 本发明还涉及除了使用用于分离塑料的酯酶，特别是聚酯化合物之外，酯酶用于防止或减少和/或防止起球，优选在纺织品，特别是塑料纤维，更优选聚酯纤维中 。 本发明还涉及新的酯酶和足够相关的蛋白质及其衍生物，含有它们的试剂及其用途。

    FLUORESCENT PROTEINS, THEIR PRODUCTION AND USE
    6.
    发明申请
    FLUORESCENT PROTEINS, THEIR PRODUCTION AND USE 失效
    荧光蛋白，其生产和使用

    公开(公告)号：US20100304435A1

    公开(公告)日：2010-12-02

    申请号：US12783301

    申请日：2010-05-19

    申请人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

    发明人： Thorsten Eggert ,  Sascha Hausmann ,  Michael Puls

    IPC分类号： C07K14/00 ,  C07H21/04 ,  C12P21/00

    CPC分类号： C07K14/21 ,  C07K14/32 ,  Y02A50/473

    摘要： The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.

    摘要翻译： 本发明提供荧光蛋白的变体，其在其用作报告蛋白和/或分析中的性质方面得到改进。 特别地，提供荧光蛋白的变体，其发荧光更亮，显示改善的量子产率和/或具有偏移的激发或发射光谱。 根据本发明的荧光蛋白质除了用不与FMN共价结合至少一个另外的点突变的氨基酸取代半胱氨酸之外，还包含其LOV结构域。

    (R)-HYDROXYNITRILE LYASE FROM BRASSICACEAE
    7.
    发明申请
    (R)-HYDROXYNITRILE LYASE FROM BRASSICACEAE 审中-公开
    （R）-HYDROXYNITRILE LYASE从BRASSICACEAE

    公开(公告)号：US20100112648A1

    公开(公告)日：2010-05-06

    申请号：US12517978

    申请日：2007-12-10

    申请人： Thorsten Eggert ,  Jennifer Andexer

    发明人： Thorsten Eggert ,  Jennifer Andexer

    IPC分类号： C12P13/00 ,  C12N9/88 ,  C12N9/14 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

    CPC分类号： C12N9/88 ,  C12P13/004

    摘要： The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.

    摘要翻译： 本发明涉及一种多肽，其可从十字花科科中分离并且至少具有羟基腈裂解酶（HNL）的活性。 本发明的羟基腈裂解酶是来自十字花科科的第一种HNL。 这种酶或其基因被分离的植物（拟南芥）也被描述为非氰化的。 到目前为止描述的所有含有HNL的植物都是氰基植物，因此迄今为止已经假设只有氰基植物含有羟基腈裂解酶。 令人惊奇的是，发现本发明的多肽（AtHNL）是（R）选择性的。 氨基酸序列为AtHNL亚基的理论分子量为29.2 kDa。 约30kDa的蛋白质的计算分子量可以通过SDS凝胶电泳确认。

    Use of esterases for separating plastics
    9.
    发明申请
    Use of esterases for separating plastics 失效
    使用酯酶分离塑料

    公开(公告)号：US20090258406A1

    公开(公告)日：2009-10-15

    申请号：US11989927

    申请日：2006-08-03

    申请人： Andreas Michels ,  André Pütz ,  Karl-Heinz Maurer ,  Thorsten Eggert ,  Karl-Erich Jäger

    发明人： Andreas Michels ,  André Pütz ,  Karl-Heinz Maurer ,  Thorsten Eggert ,  Karl-Erich Jäger

    IPC分类号： D06M16/00 ,  C12N9/16

    CPC分类号： C12N9/18

    摘要： The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof.

    摘要翻译： 本发明涉及含有酯酶的试剂，以及其用于修整纤维的用途，特别是人造纤维，包含酯酶的洗涤和清洁剂以及相应的洗涤和清洁方法，以及额外的技术应用领域。 本发明还涉及除了使用用于分离塑料的酯酶，特别是聚酯化合物之外，酯酶用于防止或减少和/或防止起球，优选在纺织品，特别是塑料纤维，更优选聚酯纤维中 。 本发明还涉及新的酯酶和足够相关的蛋白质及其衍生物，含有它们的试剂及其用途。