公开(公告)号：US20090004215A1

公开(公告)日：2009-01-01

申请号：US11202864

申请日：2005-08-12

Applicant: Rakesh Tuli ,  Samir Vishwanath Sawant ,  Shadma Ashraf ,  Pradhyumna Kumar Singh ,  Dinesh Kumar Yadav ,  Md. Shahnawaz ,  Satish Mishra

Inventor： Rakesh Tuli ,  Samir Vishwanath Sawant ,  Shadma Ashraf ,  Pradhyumna Kumar Singh ,  Dinesh Kumar Yadav ,  Md. Shahnawaz ,  Satish Mishra

IPC: A61K39/205 ,  C07K14/145 ,  C12N15/47 ,  C12N15/82 ,  A61P31/14

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/205 ,  A61K2039/517 ,  C07K2319/02 ,  C07K2319/04 ,  C07K2319/21 ,  C07K2319/50 ,  C12N15/8258 ,  C12N2760/20122 ,  C12N2760/20134

Abstract: A novel chimeric protein of rabies virus designed to express a chimeric G protein at a high level in transgenic plants. A gene was also designed and chemically synthesised to encode the chimeric G protein and expressed at high level in plant tissue. The gene was expressed in transgenic tobacco plants to examine its therapeutic efficacy against infection by rabies virus. The chimeric G protein was enriched in plant membranes. The BalbC mice were immunised with the plant leaf expressed G-protein. Plant derived chimeric G protein elicited higher immune response as compared to the commercial vaccine. The mice displayed protective immunity when they were challenged with live virus. Chimeric G protein expressed at high level in plant leaves was demonstrated to function as a commercially valuable subunit vaccine against rabies virus infection.

Abstract translation: 一种设计用于在转基因植物中高水平表达嵌合G蛋白的狂犬病病毒的新型嵌合蛋白。 还设计并化学合成基因以编码嵌合G蛋白并在植物组织中以高水平表达。 该基因在转基因烟草植物中表达，以检测其针对狂犬病病毒感染的治疗效果。 嵌合G蛋白在植物膜中富集。 用植物叶表达的G蛋白免疫BalbC小鼠。 与商业疫苗相比，植物衍生的嵌合G蛋白引起更高的免疫应答。 当用活病毒攻击时，小鼠显示出保护性免疫力。 证明在植物叶中高水平表达的嵌合G蛋白作为抗狂犬病毒感染的商业上有价值的亚单位疫苗起作用。

公开(公告)号：US07901691B2

公开(公告)日：2011-03-08

申请号：US11202864

申请日：2005-08-12

Applicant: Rakesh Tuli ,  Samir Vishwanath Sawant ,  Shadma Ashraf ,  Pradhyumna Kumar Singh ,  Dinesh Kumar Yadav ,  Mohammad Shahnawaz ,  Satish Mishra

Inventor： Rakesh Tuli ,  Samir Vishwanath Sawant ,  Shadma Ashraf ,  Pradhyumna Kumar Singh ,  Dinesh Kumar Yadav ,  Mohammad Shahnawaz ,  Satish Mishra

IPC: A61K39/205 ,  C12N15/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/205 ,  A61K2039/517 ,  C07K2319/02 ,  C07K2319/04 ,  C07K2319/21 ,  C07K2319/50 ,  C12N15/8258 ,  C12N2760/20122 ,  C12N2760/20134

Abstract: A novel chimeric protein of rabies virus designed to express a chimeric G protein at a high level in transgenic plants. A gene was also designed and chemically synthesised to encode the chimeric G protein and expressed at high level in plant tissue. The gene was expressed in transgenic tobacco plants to examine its therapeutic efficacy against infection by rabies virus. The chimeric G protein was enriched in plant membranes. The BalbC mice were immunised with the plant leaf expressed G-protein. Plant derived chimeric G protein elicited higher immune response as compared to the commercial vaccine. The mice displayed protective immunity when they were challenged with live virus. Chimeric G protein expressed at high level in plant leaves was demonstrated to function as a commercially valuable subunit vaccine against rabies virus infection.

Abstract translation: 一种设计用于在转基因植物中高水平表达嵌合G蛋白的狂犬病病毒的新型嵌合蛋白。 还设计并化学合成基因以编码嵌合G蛋白并在植物组织中以高水平表达。 该基因在转基因烟草植物中表达，以检测其针对狂犬病病毒感染的治疗效果。 嵌合G蛋白在植物膜中富集。 用植物叶表达的G蛋白免疫BalbC小鼠。 与商业疫苗相比，植物衍生的嵌合G蛋白引起更高的免疫应答。 当用活病毒攻击时，小鼠显示出保护性免疫力。 证明在植物叶中高水平表达的嵌合G蛋白作为抗狂犬病毒感染的商业上有价值的亚单位疫苗起作用。