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公开(公告)号：US20100323362A1
公开(公告)日：2010-12-23
申请号：US12821608
申请日：2010-06-23
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.
摘要翻译： 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 23S rRNA或其基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些寡聚体包括标签区，靶闭合区，启动子序列和/或结合部分。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和制药产品。
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公开(公告)号：US20090305293A1
公开(公告)日：2009-12-10
申请号：US12539518
申请日：2009-08-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.
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公开(公告)号：US09109263B2
公开(公告)日：2015-08-18
申请号：US12843736
申请日：2010-07-26
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针和对白色念珠菌26S rRNA序列或编码26S rRNA的DNA特异的检测探针。 公开了通过使用所公开的组合物在包括26S rRNA序列的体外核酸扩增或编码26S rRNA序列的DNA以产生可检测的扩增产物的方法中来检测样品中白色念珠菌存在的方法。
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4.发明授权Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa 有权用于检测和/或监测铜绿假单胞菌的组合物，试剂盒和相关方法公开(公告)号：US08927703B2
公开(公告)日：2015-01-06
申请号：US12061438
申请日：2008-04-02
CPC分类号： C12Q1/689
摘要： The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.
摘要翻译： 本发明提供了用于铜绿假单胞菌物种特异性检测的组合物，方法和试剂盒。
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公开(公告)号：US08609829B2
公开(公告)日：2013-12-17
申请号：US12624233
申请日：2009-11-23
申请人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Goslow
发明人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Goslow
CPC分类号： C12Q1/689 , C12Q1/686 , Y02A50/451
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Legionella pneumophila 16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence of L. pneumophila in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针，以及针对嗜肺军团菌16S或23S rRNA序列或编码16S或23S rRNA的DNA特异的检测探针。 公开了用于通过使用所公开的组合物在包括体外核酸扩增16S rRNA序列或编码16S rRNA序列的DNA或编码23S rRNA序列或编码 23S rRNA序列以产生可检测的扩增产物。
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6.发明申请公开(公告)号：US20120052496A1
公开(公告)日：2012-03-01
申请号：US13128384
申请日：2009-11-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/158 , Y02A50/451
摘要： The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid from Campylobacter species jejuni, coli and/or lari. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.
摘要翻译： 所公开的发明涉及包含一种或多种靶向来自空肠弯曲杆菌，大肠杆菌和/或乳杆菌的16S rRNA靶核酸的低聚物的组合物，试剂盒和方法。 组合物包括扩增寡聚物，检测探针低聚物和/或靶捕获低聚物。 试剂盒和方法包括这些寡聚物中的至少一种。
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7.发明申请COMPOSITIONS AND METHODS TO DETECT LEGIONELLA PNEUMOPHILA NUCLEIC ACID 有权组合物和检测LEGIONELLA PNEUMOPHILA核酸的方法公开(公告)号：US20100216141A1
公开(公告)日：2010-08-26
申请号：US12624233
申请日：2009-11-23
申请人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Goslow
发明人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Goslow
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/686 , Y02A50/451
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Legionella pneumophila 16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence of L. pneumophila in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针，以及针对嗜肺军团菌16S或23S rRNA序列或编码16S或23S rRNA的DNA特异的检测探针。 公开了用于通过使用所公开的组合物在包括体外核酸扩增16S rRNA序列或编码16S rRNA序列的DNA或编码23S rRNA序列或编码 23S rRNA序列以产生可检测的扩增产物。
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公开(公告)号：US07670780B2
公开(公告)日：2010-03-02
申请号：US12539518
申请日：2009-08-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针和对白色念珠菌26S rRNA序列或编码26S rRNA的DNA特异的检测探针。 公开了通过使用所公开的组合物在包括26S rRNA序列的体外核酸扩增或编码26S rRNA序列的DNA以产生可检测的扩增产物的方法中来检测样品中白色念珠菌存在的方法。
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9.发明申请COMPOSITIONS AND METHODS FOR DETECTION OF PROPIONIBACTERIUM ACNES NUCLEIC ACID 审中-公开用于检测丙酸酸核酸的组合物和方法公开(公告)号：US20090269764A1
公开(公告)日：2009-10-29
申请号：US12389350
申请日：2009-02-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： Methods for amplifying and detecting Propionibacterium acnes nucleic acid by targeting specific sequences in 16S rRNA, 23S rRNA, or DNA encoding 16S rRNA or 23S rRNA are disclosed. Nucleic acid oligonucleotide sequence compositions specific for P. acnes nucleic acid sequences in 16S or 23S rRNA or DNA encoding 16S or 23S rRNA sequences are disclosed, which are useful for amplification oligonucleotides, capture probes in sample preparation, and probes for detection of P. acnes nucleic acid sequences.
摘要翻译： 公开了通过靶向16S rRNA，23S rRNA或编码16S rRNA或23S rRNA的DNA的特异性序列来扩增和检测痤疮丙酸杆菌核酸的方法。 公开了对16S或23S rRNA或编码16S或23S rRNA序列的编码16S或23S rRNA序列的痤疮丙酸杆菌核酸序列特异性的核酸寡核苷酸序列组合物，其可用于扩增寡核苷酸，样品制备中的捕获探针和用于检测痤疮丙酸杆菌 核酸序列。
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10.发明申请COMPOSITIONS, KITS AND RELATED METHODS FOR THE DETECTION AND/OR MONITORING OF PSEUDOMONAS AERUGINOSA 有权用于检测和/或监测PSEUDOMONAS AERUGINOSA的组合物，试剂盒和相关方法
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