公开(公告)号：US5262333A

公开(公告)日：1993-11-16

申请号：US898238

申请日：1992-06-12

申请人： Daniel F. Heiman ,  Sharon A. Johnson ,  Hsiang-Yun Y. Hu

发明人： Daniel F. Heiman ,  Sharon A. Johnson ,  Hsiang-Yun Y. Hu

IPC分类号： G01N33/542 ,  G01N33/94

CPC分类号： G01N33/946 ,  G01N33/542 ,  Y10S436/816

摘要： This disclosure relates to a method and reagents for determining amphetamine and d-methamphetamine in a biological fluid, such as urine. In particular, this disclosure relates to improvements in a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and d-methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross reactants such as .beta.-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH, and contacting the sample with riboflavin binding protein to reduce interference from fluorescent components in the sample. The procedure also maintains the cross reactivity of the immunoassay for tyramine at about 0.4% and for 1-methamphetamine below about 5.1% and eliminates the necessity of using controlled substances as starting materials.

摘要翻译： 本公开涉及用于在生物流体例如尿中测定苯丙胺和d-甲基苯丙胺的方法和试剂。 特别地，本公开涉及在单次测定中用于确定苯丙胺和d-甲基苯丙胺的存在的荧光偏振免疫测定方法的改进，以及在这种方法中用作试剂的新型示踪剂化合物。 所述方法包括通过预先处理生物样品以消除交叉反应物例如β-羟基苯乙胺，通过仅用pH约4.0至约7.5的高碘酸钠溶液预温育样品，而不调节至碱性pH，并将样品与核黄素接触 结合蛋白以减少样品中荧光成分的干扰。 该方法还保持酪胺的免疫测定在约0.4％的交叉反应性和低于约5.1％的1-甲基苯丙胺，并且不需要使用受控物质作为起始原料。