公开(公告)号：US20180011079A1

公开(公告)日：2018-01-11

申请号：US15611327

申请日：2017-06-01

申请人： Healgen Scientific Limited ,  Zhejiang Orient Gene Biotech Co., LTD.

发明人： Jianqiu Fang ,  Siyu Lei

IPC分类号： G01N33/493 ,  A61B10/00 ,  B01L3/00 ,  G01N33/94

CPC分类号： G01N33/493 ,  A61B10/007 ,  A61B10/0096 ,  A61B2010/0003 ,  A61B2010/0009 ,  B01L3/502 ,  B01L3/508 ,  B01L3/50853 ,  B01L2200/025 ,  B01L2200/141 ,  B01L2200/143 ,  B01L2200/145 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0609 ,  B01L2300/0627 ,  B01L2300/0663 ,  B01L2300/0825 ,  B01L2300/0832 ,  B01L2300/0848 ,  B01L2300/123 ,  B65D41/0471 ,  G01N33/94 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9486

摘要： The present invention provides an apparatus for detecting the presence or absence of an analyte in liquid sample, including: a collection chamber, including an opening for collecting a liquid sample; a testing element for testing the analyte in liquid sample; and a cover for covering the opening of the collection chamber; wherein the apparatus further includes a prompting device for prompting if the cover is covered to a specified location, and the prompting device shall at least includes a first element and a second element, the first element is in contact with the second element, wherein one element vibrates to produce a sound. In some preferred ways, the second element produces friction with the first element, to cause one of the elements to generate vibrations. The apparatus in the present invention can allow the prompting sound to be clear and loud.

公开(公告)号：US09804178B2

公开(公告)日：2017-10-31

申请号：US14930242

申请日：2015-11-02

申请人： Daniel Roncancio ,  Haixiang Yu ,  Xiaowen Xu ,  Yi Xiao

发明人： Daniel Roncancio ,  Haixiang Yu ,  Xiaowen Xu ,  Yi Xiao

IPC分类号： C07H21/02 ,  C07H21/04 ,  G01N33/94 ,  C12N15/115 ,  A61K31/70

CPC分类号： G01N33/946 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10

摘要： The invention pertains to a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements based on an aptamer sensor that reports the presence of cocaine via the displacement and unquenching of a bound fluorophore molecule. In certain embodiments, the invention provides novel aptamers, which have reduced background fluorescence, bind a fluorophore molecule tightly, and show an increased signal gain in the presence of cocaine.

公开(公告)号：US09599628B2

公开(公告)日：2017-03-21

申请号：US15064532

申请日：2016-03-08

申请人： University of Utah Research Foundation

发明人： Jennifer M. Heemstra

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/94 ,  G01N33/74 ,  C12N15/115

CPC分类号： G01N33/9486 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/10 ,  G01N33/53 ,  G01N33/5308 ,  G01N33/743 ,  G01N33/94 ,  G01N33/946 ,  G01N2400/00

摘要： Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.

公开(公告)号：US20160375131A1

公开(公告)日：2016-12-29

申请号：US15189930

申请日：2016-06-22

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Yung Chang ,  Sidney Hecht ,  Joseph Leal ,  Viswanath Arutla ,  Xiaowei Liu ,  Sriram Sokalingam ,  Paul Pentel

IPC分类号： A61K39/385 ,  G01N33/94 ,  G06F19/16 ,  G06F19/18 ,  C07D401/14 ,  A61K47/48

CPC分类号： A61K39/385 ,  A61K39/0013 ,  A61K47/646 ,  A61K2039/6012 ,  C07D401/14 ,  G01N33/946 ,  G06F19/16 ,  G06F19/18

摘要： Provided herein are methods for rational design of nicotine haptens. More particularly, provided herein are methods for designing, selecting, and synthesizing nicotine haptens and nicotine hapten conjugates. Also provided herein are novel nicotine haptens and methods for using nicotine haptens to treat nicotine addiction.

摘要翻译： 本文提供了尼古丁半抗原合理设计的方法。 更具体地，本文提供了用于设计，选择和合成尼古丁半抗原和尼古丁半抗原共轭物的方法。 本文还提供了新型尼古丁半抗原以及使用尼古丁半抗原来治疗尼古丁成瘾的方法。

公开(公告)号：US09279805B2

公开(公告)日：2016-03-08

申请号：US14288240

申请日：2014-05-27

申请人： University of Utah Research Foundation

发明人： Jennifer M. Heemstra

IPC分类号： C12N15/115 ,  G01N33/53 ,  G01N33/94 ,  G01N33/74

CPC分类号： G01N33/9486 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/10 ,  G01N33/53 ,  G01N33/5308 ,  G01N33/743 ,  G01N33/94 ,  G01N33/946 ,  G01N2400/00

摘要： Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.

摘要翻译： 提供了用于检测小分子的方法，测定和产物。 在一个方面，例如，检测样品中的小分子的方法可以包括将具有与其偶联的第一反应性基团的DNA裂解适体的第一半一起反应，具有第二反应性基团的DNA裂解适体的第二半 其中DNA裂解适体对小分子是选择性的，以及含有小分子的样品。 上半部和第二半部分与小分子结合，第一反应基团和第二反应基团反应形成上半部和下半部分的适体连接产物。 该方法还可以包括测定适体连接产物以检测样品中的小分子存在。

公开(公告)号：US20160025752A1

公开(公告)日：2016-01-28

申请号：US14747659

申请日：2015-06-23

申请人： Seed Research and Development, LLC

发明人： Frank Santiago ,  Leanna M. Levine ,  Rafael de la Torre Fornell ,  Magin Farre Albaladejo

IPC分类号： G01N33/94

CPC分类号： G01N33/948 ,  B01L3/5023 ,  B01L2200/027 ,  B01L2300/043 ,  B01L2300/047 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0683 ,  G01N15/0612 ,  G01N21/8483 ,  G01N33/94 ,  G01N33/946 ,  G01N2015/0065 ,  G01N2021/8488 ,  G01N2035/00108 ,  G08C17/02

摘要： The present disclosure relates to devices and methods for determining a target analyte in a sample (e.g., a bodily fluid sample such as sweat, blood, urine, lavage, plasma, saliva, tears) using an analysis device (e.g., a bodily fluid analysis device). The device may include a lateral flow assay in some embodiments. Certain embodiments involve determining a concentration of a target analyte in a sample by generating a volumetric calculation of the sample and/or by use of a non-target analyte. The devices and methods can be used for obtaining and processing a sample to determine the presence and/or concentration of such analytes. In some cases, the methods and/or devices can be used to determine impairment (e.g., drug impairment) in a subject.

摘要翻译： 本公开涉及使用分析装置（例如，体液分析法）来确定样品中的目标分析物（例如，体液样品，例如汗液，血液，尿液，灌洗液，血浆，唾液，眼泪）的装置和方法 设备）。 在一些实施方案中，该装置可以包括侧流测定。 某些实施方案涉及通过产生样品的体积计算和/或通过使用非目标分析物来确定样品中目标分析物的浓度。 这些装置和方法可用于获得和处理样品以确定这些分析物的存在和/或浓度。 在某些情况下，方法和/或装置可用于确定受试者的损伤（例如药物损伤）。

公开(公告)号：US20150346226A1

公开(公告)日：2015-12-03

申请号：US14723724

申请日：2015-05-28

申请人： Randox Laboratories Limited

发明人： Ivan Robert McConnell ,  Elouard Benchikh ,  Peter Fitzgerald ,  Andrew Philip Lowry

IPC分类号： G01N33/94 ,  C07K16/44

CPC分类号： G01N33/94 ,  C07K16/44 ,  C07K2317/30 ,  C07K2317/33 ,  G01N33/946 ,  G01N2430/00

摘要： An immunoassay method for detecting and determining ‘NBOMe’ family designer drugs is described. Also described are components for use in implementing the method, namely, antibodies, detection agents, solid state devices and kits as well as immunogens used to raise the antibodies.

摘要翻译： 描述了用于检测和确定“NBOMe”家族设计药物的免疫测定方法。 还描述了用于实施该方法的组分，即抗体，检测剂，固态装置和试剂盒以及用于产生抗体的免疫原。

公开(公告)号：US20150247874A1

公开(公告)日：2015-09-03

申请号：US14193673

申请日：2014-02-28

申请人： Government of the United States as Represented by the Secretary of the Air Force

发明人： Jorge Luis Chavez Benavides ,  Joshua Smith ,  Nancy Kelley-Loughnane ,  Joshua Hagen ,  Peter A. Mirau ,  Rajesh Naik

IPC分类号： G01N33/94

CPC分类号： G01N33/946 ,  G01N33/587 ,  Y10S977/774 ,  Y10S977/81 ,  Y10S977/902

摘要： A method of detecting a presence of an analyte in a sample. The method includes selecting an aptamer selective to the analyte and binding the aptamer to a nanoparticle. The nanoparticles having a free state are perceived as a first color and an aggregate state are perceived as a second color. The sample is introduced to the nanoparticle-bound aptamers, and aggregation of the nanoparticles is promoted. A colorimetric change is analyzed, wherein the aggregate state of the nanoparticles is achieved in the presence of the analyte in the sample.

摘要翻译： 检测样品中分析物的存在的方法。 该方法包括选择对分析物选择性的适体和将适配子结合到纳米颗粒上。 具有自由状态的纳米颗粒被感知为第一颜色，聚集状态被感知为第二颜色。 将样品引入到纳米颗粒结合的适体中，并促进纳米颗粒的聚集。 分析比色变化，其中纳米颗粒的聚集状态在样品中分析物存在下实现。

公开(公告)号：US20150024514A1

公开(公告)日：2015-01-22

申请号：US14383149

申请日：2013-03-06

申请人： SECURETEC DETEKTIONS-SYSTEME AG

发明人： Torsten Stadthagen ,  Frank Schwieger ,  Sebastian Klaus

IPC分类号： G01N33/94

CPC分类号： G01N33/948 ,  G01N33/558 ,  G01N33/946

摘要： The present invention relates to a method for determining analytes and to a device suitable for this purpose.

摘要翻译： 本发明涉及一种用于确定分析物的方法以及适用于该目的的装置。

公开(公告)号：US20140179024A1

公开(公告)日：2014-06-26

申请号：US14191043

申请日：2014-02-26

申请人： Jeroen Hans NIEUWENHUIS ,  Ahmed Mohammed Taki JEHANLI ,  Femke Karina DE THEIJE ,  Eduard Gerard Marie PELSSERS

发明人： Jeroen Hans NIEUWENHUIS ,  Ahmed Mohammed Taki JEHANLI ,  Femke Karina DE THEIJE ,  Eduard Gerard Marie PELSSERS

IPC分类号： G01N33/94

CPC分类号： G01N33/9486 ,  B01L3/502753 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/54393 ,  G01N33/946

摘要： The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogues.

摘要翻译： 本发明提供了一种用于检测唾液中的药物或其他化合物的装置。 因此，本发明提供了一种用于检测唾液样品中一种或多种分析物的存在的装置，其包括：（a）用于特异性或非特异性地去除唾液样品的至少一部分部分的一个或多个预处理区域 干扰一种或多种分析物的检测; 和（b）包含生物传感器表面的检测区域，所述表面包括：能够特异性结合所述一种或多种分析物的分子; 或一种或多种分析物和/或分析物类似物。