公开(公告)号：US08865969B2

公开(公告)日：2014-10-21

申请号：US13008487

申请日：2011-01-18

申请人： Satoshi Kondo ,  Chikara Ohto ,  Kunihiro Ohta ,  Shuichi Ohsato ,  Norihiro Mitsukawa ,  Nobuhiko Muramoto ,  Hiroki Sugimoto

发明人： Satoshi Kondo ,  Chikara Ohto ,  Kunihiro Ohta ,  Shuichi Ohsato ,  Norihiro Mitsukawa ,  Nobuhiko Muramoto ,  Hiroki Sugimoto

IPC分类号： C12N15/87 ,  C12N15/05

CPC分类号： C12N15/8213

摘要： According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.

摘要翻译： 根据本发明，建立了增加植物基因组DNA中基因重组频率的技术。 这样的技术包括：将能够在靶植物细胞中表达的限制酶基因引入植物细胞，并使限制酶基因瞬时表达，以诱导基因组DNA的基因重组; 或使用农杆菌方法引入启动子和限制酶基因，以诱导基因组DNA的遗传重组。

公开(公告)号：US20110277189A1

公开(公告)日：2011-11-10

申请号：US13008487

申请日：2011-01-18

申请人： Satoshi KONDO ,  Chikara OHTO ,  Kunihiro OHTA ,  Shuichi OHSATO ,  Norihiro MITSUKAWA ,  Nobuhiko MURAMOTO ,  Hiroki SUGIMOTO

发明人： Satoshi KONDO ,  Chikara OHTO ,  Kunihiro OHTA ,  Shuichi OHSATO ,  Norihiro MITSUKAWA ,  Nobuhiko MURAMOTO ,  Hiroki SUGIMOTO

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N15/8213

摘要： According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.

摘要翻译： 根据本发明，建立了增加植物基因组DNA中基因重组频率的技术。 这样的技术包括：将能够在靶植物细胞中表达的限制酶基因引入植物细胞，并使限制酶基因瞬时表达，以诱导基因组DNA的基因重组; 或使用农杆菌方法引入启动子和限制酶基因，以诱导基因组DNA的遗传重组。