公开(公告)号：US20130142813A1

公开(公告)日：2013-06-06

申请号：US13808475

申请日：2011-07-07

申请人： Yoshio Hirabayashi ,  Hiroyuki Kamiguchi ,  Kunihiro Ohta ,  Akiho Nakamura

发明人： Yoshio Hirabayashi ,  Hiroyuki Kamiguchi ,  Kunihiro Ohta ,  Akiho Nakamura

IPC分类号： C07K16/18

CPC分类号： C07K16/18 ,  A61K2039/505 ,  C07K2317/76 ,  C07K2317/92

摘要： In order to identify a factor demonstrating a repellent effect on axon extension of a neuron, lysophosphatidylglucoside (LPG) has been found to have an activity as a chemorepellent effect on axon guidance of a DRG sensory neuron specific to a neuron expressing TrkA, an NGF receptor.In addition, it has been found out that suppressing the function of LPG makes it possible to suppress a repellent effect of LPG on axon extension of a neuron expressing TrkA. A molecule capable of suppressing the function of LPG can be a composition promoting repairing of a neural circuit in neuronal damages and the like.

摘要翻译： 为了鉴定表现出对神经元轴突延伸的驱避作用的因子，已经发现溶血磷脂酰葡萄糖苷（LPG）具有作为对表达TrkA，NGF受体的神经元特异性的DRG感觉神经元的轴突引导的化学诱导作用的活性 。 此外，已经发现抑制LPG的功能使得可以抑制LPG对表达TrkA的神经元的轴突延伸的排斥作用。 能够抑制LPG功能的分子可以是促进神经元损伤等中神经回路修复的组合物。

公开(公告)号：US07776599B2

公开(公告)日：2010-08-17

申请号：US10522644

申请日：2003-07-28

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

IPC分类号： C12N15/09 ,  C12N15/85 ,  C12N5/0781

CPC分类号： C07K16/42 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/23 ,  C07K2317/565 ,  C12N15/907

摘要： The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes.By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact and the like with histone acetylase inhibitor and the like (for example, trichostatin A and the like), thereby relaxing the chromatin structure at said antibody locus, somatic homologous recombination at an antibody locus is enhanced, and the production of diverse antibody molecules is made possible.The production of antibodies that bind specifically to antigens from cell populations in which the antibody molecules have been diversified by the enhancement of somatic homologous recombination is made possible by using an appropriate selection method (for example, beads coated with antigen and the like).

摘要翻译： 本发明提供了一种在免疫细胞中抗体基因座上显着增强体细胞同源重组的结果，获得多种抗体的新方法。 通过将抗体基因座（例如DT40细胞等）中发生DNA同源重组的免疫细胞与组蛋白乙酰酶抑制剂等（例如曲古抑菌素A等）接触等，从而使 所述抗体基因座处的染色质结构，抗体基因座上的体细胞同源重组被增强，并且使各种抗体分子的产生成为可能。 通过使用适当的选择方法（例如，用抗原等涂布的珠子），可以通过增强体细胞同源重组来产生与抗体分子多样化的来自细胞群体的抗原的抗体的产生。

公开(公告)号：US20060183225A1

公开(公告)日：2006-08-17

申请号：US10522644

申请日：2003-07-28

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

IPC分类号： C12N15/87 ,  C12N5/06 ,  A61K31/19 ,  C07K16/18

CPC分类号： C07K16/42 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/23 ,  C07K2317/565 ,  C12N15/907

摘要： The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes. By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact and the like with histone acetylase inhibitor and the like (for example, trichostatin A and the like), thereby relaxing the chromatin structure at said antibody locus, somatic homologous recombination at an antibody locus is enhanced, and the production of diverse antibody molecules is made possible. The production of antibodies that bind specifically to antigens from cell populations in which the antibody molecules have been diversified by the enhancement of somatic homologous recombination is made possible by using an appropriate selection method (for example, beads coated with antigen and the like)

摘要翻译： 本发明提供了一种在免疫细胞中抗体基因座上显着增强体细胞同源重组的结果，获得多种抗体的新方法。 通过将抗体基因座（例如DT40细胞等）中发生DNA同源重组的免疫细胞与组蛋白乙酰酶抑制剂等（例如曲古抑菌素A等）接触等，从而使 所述抗体基因座处的染色质结构，抗体基因座上的体细胞同源重组被增强，并且使各种抗体分子的产生成为可能。 通过使用适当的选择方法（例如，用抗原等包被的珠粒），可以通过增强体细胞同源重组使来自细胞群体的抗原特异性结合抗体分子多样化的抗体的产生成为可能，

公开(公告)号：US20110070650A1

公开(公告)日：2011-03-24

申请号：US12813454

申请日：2010-06-10

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

IPC分类号： C12N15/01 ,  C12N5/0781

CPC分类号： C07K16/42 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/23 ,  C07K2317/565 ,  C12N15/907

摘要： The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes.By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact and the like with histone acetylase inhibitor and the like (for example, trichostatin A and the like), thereby relaxing the chromatin structure at said antibody locus, somatic homologous recombination at an antibody locus is enhanced, and the production of diverse antibody molecules is made possible.The production of antibodies that bind specifically to antigens from cell populations in which the antibody molecules have been diversified by the enhancement of somatic homologous recombination is made possible by using an appropriate selection method (for example, beads coated with antigen and the like)

摘要翻译： 本发明提供了一种在免疫细胞中抗体基因座上显着增强体细胞同源重组的结果，获得多种抗体的新方法。 通过将抗体基因座（例如DT40细胞等）中发生DNA同源重组的免疫细胞与组蛋白乙酰酶抑制剂等（例如曲古抑菌素A等）接触等，从而使 所述抗体基因座处的染色质结构，抗体基因座上的体细胞同源重组被增强，并且使各种抗体分子的产生成为可能。 通过使用适当的选择方法（例如，用抗原等包被的珠粒），可以通过增强体细胞同源重组使来自细胞群体的抗原特异性结合抗体分子多样化的抗体的产生成为可能，

公开(公告)号：US20100311608A1

公开(公告)日：2010-12-09

申请号：US12777562

申请日：2010-05-11

申请人： Hiroyuki OSADA ,  Kaori HONDA ,  Kunihiro OHTA ,  Akiho MURAYAMA

发明人： Hiroyuki OSADA ,  Kaori HONDA ,  Kunihiro OHTA ,  Akiho MURAYAMA

IPC分类号： C40B30/04 ,  B05D3/06 ,  C40B40/00

CPC分类号： G01N33/54326 ,  B03C1/01 ,  B03C2201/18 ,  G01N33/54353

摘要： It is an object of the present invention to provide a method for stably and efficiently binding a compound to magnetic beads. The present invention relates to a method for producing compound-bound magnetic beads, which comprises: allowing a compound to come into contact with magnetic beads, on the surface of each of which a photoreactive compound has bound; extending the magnetic beads together with the compound on a support; and applying light to the magnetic beads to form a covalent bond between the photoreactive compound and the compound.

摘要翻译： 本发明的目的是提供一种稳定且有效地将化合物与磁珠结合的方法。 本发明涉及一种化合物结合磁珠的制造方法，其特征在于：使化合物在光反应性化合物结合的表面上与磁珠接触; 将磁珠与化合物一起在载体上延伸; 并向光磁珠施加光，以在光反应性化合物和化合物之间形成共价键。

公开(公告)号：US20080166809A1

公开(公告)日：2008-07-10

申请号：US11791126

申请日：2005-11-22

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Kouji Hirota ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Kouji Hirota ,  Takehiko Shibata

IPC分类号： C12N15/01 ,  C12N1/00

CPC分类号： C12N15/905 ,  C12N9/16 ,  C12N15/81

摘要： The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.

摘要翻译： 本发明涉及提高基因组DNA中基因重组频率的方法和诱导基因组重排的方法。 具体地说，根据本发明，提供了在基因组中的任何位置发生遗传重组的细胞中增加遗传重组频率的方法，包括使限制性内切酶在细胞中表达，引起瞬时激活 限制酶，然后将2个以上的双链裂解引入细胞的任何基因组DNA，以增加遗传重组频率; 通过使用上述方法诱导基因组重排的方法; 每个细胞通过使用上述2种方法制备。

公开(公告)号：US08591902B2

公开(公告)日：2013-11-26

申请号：US13808475

申请日：2011-07-07

申请人： Yoshio Hirabayashi ,  Hiroyuki Kamiguchi ,  Kunihiro Ohta ,  Akiho Nakamura

发明人： Yoshio Hirabayashi ,  Hiroyuki Kamiguchi ,  Kunihiro Ohta ,  Akiho Nakamura

IPC分类号： C07K16/18 ,  A61K31/7028 ,  G01N33/53

CPC分类号： C07K16/18 ,  A61K2039/505 ,  C07K2317/76 ,  C07K2317/92

摘要： In order to identify a factor demonstrating a repellent effect on axon extension of a neuron, lysophosphatidylglucoside (LPG) has been found to have an activity as a chemorepellent effect on axon guidance of a DRG sensory neuron specific to a neuron expressing TrkA, an NGF receptor.In addition, it has been found out that suppressing the function of LPG makes it possible to suppress a repellent effect of LPG on axon extension of a neuron expressing TrkA. A molecule capable of suppressing the function of LPG can be a composition promoting repairing of a neural circuit in neuronal damages and the like.

摘要翻译： 为了鉴定表现出对神经元轴突延伸的驱避作用的因子，已经发现溶血磷脂酰葡萄糖苷（LPG）具有作为对表达TrkA，NGF受体的神经元特异性的DRG感觉神经元的轴突引导的化学诱导作用的活性 。 此外，已经发现抑制LPG的功能使得可以抑制LPG对表达TrkA的神经元的轴突延伸的排斥作用。 能够抑制LPG功能的分子可以是促进神经元损伤等中神经回路修复的组合物。

公开(公告)号：US20080176753A1

公开(公告)日：2008-07-24

申请号：US11666562

申请日：2005-11-07

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

IPC分类号： C40B20/04 ,  C40B20/00 ,  C07K16/00

CPC分类号： C40B30/04 ,  C07K16/00 ,  C12N15/1034 ,  G01N33/54326 ,  G01N33/54393 ,  G01N33/6845 ,  G01N33/6854

摘要： The present invention relates to a method of selecting a protein specifically binding to a specific ligand.A method of selecting from a diversifying library a protein specifically binding to a ligand of interest, which comprises: a step of allowing proteins existing in the above library to come into contact with the ligand of interest, so as to select proteins binding to the ligand of interest; a step of allowing the obtained proteins to come into contact with a control ligand, so as to determine the presence or absence of the binding ability of the above proteins to the control ligand; and a step of selecting proteins that are determined not to have the binding ability to the control ligand.

摘要翻译： 本发明涉及选择与特异性配体特异性结合的蛋白质的方法。 从多样化文库中选择特异性结合目的配体的蛋白质的方法，该方法包括：使存在于上述文库中的蛋白质与感兴趣的配体接触的步骤，以便选择与配体结合的蛋白质 出于兴趣; 使获得的蛋白质与对照配体接触的步骤，以确定上述蛋白质与对照配体的结合能力的存在或不存在; 以及选择被确定为不具有与对照配体的结合能力的蛋白质的步骤。

公开(公告)号：US08865969B2

公开(公告)日：2014-10-21

申请号：US13008487

申请日：2011-01-18

申请人： Satoshi Kondo ,  Chikara Ohto ,  Kunihiro Ohta ,  Shuichi Ohsato ,  Norihiro Mitsukawa ,  Nobuhiko Muramoto ,  Hiroki Sugimoto

发明人： Satoshi Kondo ,  Chikara Ohto ,  Kunihiro Ohta ,  Shuichi Ohsato ,  Norihiro Mitsukawa ,  Nobuhiko Muramoto ,  Hiroki Sugimoto

IPC分类号： C12N15/87 ,  C12N15/05

CPC分类号： C12N15/8213

摘要： According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.

摘要翻译： 根据本发明，建立了增加植物基因组DNA中基因重组频率的技术。 这样的技术包括：将能够在靶植物细胞中表达的限制酶基因引入植物细胞，并使限制酶基因瞬时表达，以诱导基因组DNA的基因重组; 或使用农杆菌方法引入启动子和限制酶基因，以诱导基因组DNA的遗传重组。

公开(公告)号：US08187884B2

公开(公告)日：2012-05-29

申请号：US12813454

申请日：2010-06-10

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Takehiko Shibata

IPC分类号： C12N15/09 ,  C12N15/85 ,  C12N5/0781

CPC分类号： C07K16/42 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/23 ,  C07K2317/565 ,  C12N15/907

摘要： The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes.By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact and the like with histone acetylase inhibitor and the like (for example, trichostatin A and the like), thereby relaxing the chromatin structure at said antibody locus, somatic homologous recombination at an antibody locus is enhanced, and the production of diverse antibody molecules is made possible.The production of antibodies that bind specifically to antigens from cell populations in which the antibody molecules have been diversified by the enhancement of somatic homologous recombination is made possible by using an appropriate selection method (for example, beads coated with antigen and the like).

摘要翻译： 本发明提供了一种在免疫细胞中抗体基因座上显着增强体细胞同源重组的结果，获得多种抗体的新方法。 通过将抗体基因座（例如DT40细胞等）中发生DNA同源重组的免疫细胞与组蛋白乙酰酶抑制剂等（例如曲古抑菌素A等）接触等，从而使 所述抗体基因座处的染色质结构，抗体基因座上的体细胞同源重组被增强，并且使各种抗体分子的产生成为可能。 通过使用适当的选择方法（例如，用抗原等涂布的珠子），可以通过增强体细胞同源重组来产生与抗体分子多样化的来自细胞群体的抗原的抗体的产生。