公开(公告)号：US06197229B1

公开(公告)日：2001-03-06

申请号：US09209032

申请日：1998-12-10

申请人： Shuicho Ando ,  David Putnam ,  Robert S. Langer

发明人： Shuicho Ando ,  David Putnam ,  Robert S. Langer

IPC分类号： B01J1302

CPC分类号： A61K48/00 ,  A61K9/1617 ,  A61K9/1623 ,  A61K9/1647 ,  A61K47/183 ,  A61K47/26

摘要： A method for formulation of high supercoiled DNA content microspheres is described herein. A primary emulsion is formed which optionally contains a DNA nicking inhibitor in addition to DNA with or without buffer. The temperature of the primary emulsion is lowered below the freezing point of the aqueous inner phase which provides increased encapsulation efficiency by decreasing the rate of diffusion of DNA out of the aqueous phase. Thereafter, the primary emulsion is transferred to a water-based surfactant solution and subjected to homogenization to form a secondary microsphere emulsion. The organic phase is removed and the microspheres hardened which are then isolated, frozen and lyophilized.

摘要翻译： 本文描述了制备高超螺旋DNA含量微球的方法。 除了具有或不具有缓冲液的DNA之外，形成任选含有DNA切口抑制剂的初级乳液。 初级乳液的温度降低到内层水相的凝固点以下，通过降低DNA从水相中扩散的速率来提高包封效率。 此后，将初级乳液转移到水性表面活性剂溶液中并进行均化以形成二次微球体乳液。 除去有机相，然后将微球硬化，然后分离，冷冻并冻干。