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公开(公告)号：US06197229B1
公开(公告)日：2001-03-06
申请号：US09209032
申请日：1998-12-10
申请人： Shuicho Ando , David Putnam , Robert S. Langer
发明人： Shuicho Ando , David Putnam , Robert S. Langer
IPC分类号： B01J1302
CPC分类号： A61K48/00 , A61K9/1617 , A61K9/1623 , A61K9/1647 , A61K47/183 , A61K47/26
摘要： A method for formulation of high supercoiled DNA content microspheres is described herein. A primary emulsion is formed which optionally contains a DNA nicking inhibitor in addition to DNA with or without buffer. The temperature of the primary emulsion is lowered below the freezing point of the aqueous inner phase which provides increased encapsulation efficiency by decreasing the rate of diffusion of DNA out of the aqueous phase. Thereafter, the primary emulsion is transferred to a water-based surfactant solution and subjected to homogenization to form a secondary microsphere emulsion. The organic phase is removed and the microspheres hardened which are then isolated, frozen and lyophilized.
摘要翻译： 本文描述了制备高超螺旋DNA含量微球的方法。 除了具有或不具有缓冲液的DNA之外，形成任选含有DNA切口抑制剂的初级乳液。 初级乳液的温度降低到内层水相的凝固点以下，通过降低DNA从水相中扩散的速率来提高包封效率。 此后，将初级乳液转移到水性表面活性剂溶液中并进行均化以形成二次微球体乳液。 除去有机相，然后将微球硬化，然后分离，冷冻并冻干。
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公开(公告)号：US08557231B2
公开(公告)日：2013-10-15
申请号：US11099886
申请日：2005-04-06
IPC分类号： A61K31/785 , A61K31/765 , C08G69/08
CPC分类号： A61K47/34 , A61K9/5146 , A61K31/711 , A61K31/713 , A61K47/59 , A61K47/593 , A61K47/6929 , B82Y5/00 , C08G63/685 , C08G73/02 , C08G73/0273 , C08G73/06 , C08G73/0611 , C08G73/0616 , C12N15/88
摘要： Poly(β-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.
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公开(公告)号：US08287849B2
公开(公告)日：2012-10-16
申请号：US12507999
申请日：2009-07-23
IPC分类号： A61K31/765 , A61K9/00 , C08F26/00
CPC分类号： A61K47/34 , A61K9/5146 , A61K31/711 , A61K31/713 , A61K47/59 , A61K47/593 , A61K47/6929 , B82Y5/00 , C08G63/685 , C08G73/02 , C08G73/0273 , C08G73/06 , C08G73/0611 , C08G73/0616 , C12N15/88
摘要： Poly(β-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.
摘要翻译： 描述了从双（仲胺）或伯胺向双（丙烯酸酯）共轭加成制备的聚（＆（bgr。 - 氨基酯））。 还提供了从市售的原料制备这些聚合物的方法。 这些含叔胺的聚合物优选是可生物降解的和生物相容的并且可以用于各种药物递送系统中。 鉴于这些聚合物的聚（胺）性质，它们特别适用于递送多核苷酸。 已经制备了含有聚合物/多核苷酸复合物的纳米颗粒。 本发明的聚合物也可以用于包封待递送的其它试剂。 考虑到缓冲其周围环境的pH值，它们特别适用于提供不稳定剂。
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公开(公告)号：US20100036084A1
公开(公告)日：2010-02-11
申请号：US12507999
申请日：2009-07-23
IPC分类号： C08G63/685
CPC分类号： A61K47/34 , A61K9/5146 , A61K31/711 , A61K31/713 , A61K47/59 , A61K47/593 , A61K47/6929 , B82Y5/00 , C08G63/685 , C08G73/02 , C08G73/0273 , C08G73/06 , C08G73/0611 , C08G73/0616 , C12N15/88
摘要： Poly(β-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.
摘要翻译： 描述了从双（仲胺）或伯胺向双（丙烯酸酯）共轭加成制备的聚（β-氨基酯）。 还提供了从市售的原料制备这些聚合物的方法。 这些含叔胺的聚合物优选是可生物降解的和生物相容的并且可以用于各种药物递送系统中。 鉴于这些聚合物的聚（胺）性质，它们特别适用于递送多核苷酸。 已经制备了含有聚合物/多核苷酸复合物的纳米颗粒。 本发明的聚合物也可以用于包封待递送的其它试剂。 考虑到缓冲其周围环境的pH值，它们特别适用于提供不稳定剂。
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公开(公告)号：US06998115B2
公开(公告)日：2006-02-14
申请号：US09969431
申请日：2001-10-02
IPC分类号： A61K31/765 , C08G69/44 , C08G63/44 , C07C327/00
CPC分类号： A61K47/34 , A61K9/5146 , A61K31/711 , A61K31/713 , A61K47/59 , A61K47/593 , A61K47/6929 , B82Y5/00 , C08G63/685 , C08G73/02 , C08G73/0273 , C08G73/06 , C08G73/0611 , C08G73/0616 , C12N15/88
摘要： Poly(β-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.
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公开(公告)号：US10696944B2
公开(公告)日：2020-06-30
申请号：US14352354
申请日：2012-10-17
申请人： Massachusetts Institute of Technology , Armon R. Sharei , Andrea Adamo , Robert S. Langer , Klavs F. Jensen
摘要： A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.
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公开(公告)号：US09333179B2
公开(公告)日：2016-05-10
申请号：US12573591
申请日：2009-10-05
申请人： Liangfang Zhang , Aleksandar F. Radovic-Moreno , Frank X. Gu , Frank Alexis , Robert S. Langer , Omid C. Farokhzad
发明人： Liangfang Zhang , Aleksandar F. Radovic-Moreno , Frank X. Gu , Frank Alexis , Robert S. Langer , Omid C. Farokhzad
CPC分类号： A61K9/5123 , A61K9/5153 , A61K9/5192 , A61K47/62 , A61K47/6935 , A61K47/6937 , B82Y5/00
摘要： The present invention generally relates to nanoparticles with an amphiphilic component. One aspect of the invention is directed to a method of developing nanoparticles with desired properties. In one set of embodiments, the method includes producing libraries of nanoparticles having highly controlled properties, which can be formed by mixing together two or more macromolecules in different ratios. One or more of the macromolecules may be a polymeric conjugate of a moiety to a biocompatible polymer. In some cases, the nanoparticle may contain a drug. Other aspects of the invention are directed to methods using nanoparticle libraries.
摘要翻译： 本发明通常涉及具有两亲性组分的纳米颗粒。 本发明的一个方面涉及一种开发具有所需性质的纳米颗粒的方法。 在一组实施方案中，该方法包括制备具有高度控制性质的纳米颗粒的文库，其可通过将两种或多种不同比例的大分子混合在一起形成。 一个或多个大分子可以是部分与生物相容性聚合物的聚合物缀合物。 在一些情况下，纳米颗粒可以含有药物。 本发明的其它方面涉及使用纳米颗粒文库的方法。
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公开(公告)号：US09216188B2
公开(公告)日：2015-12-22
申请号：US12553800
申请日：2009-09-03
IPC分类号： A61K9/00 , A61K31/00 , A61K31/7052 , A61L27/26 , A61L27/38 , A61L27/52 , C08J3/075 , C08J3/24
CPC分类号： A61L27/26 , A61K31/00 , A61K31/7052 , A61L27/38 , A61L27/3834 , A61L27/50 , A61L27/52 , A61L2400/06 , A61L2430/34 , C08J3/075 , C08J3/246 , C08J2305/08 , C08J2371/02 , C08L71/02
摘要： The present invention provides hydrogels and compositions thereof for vocal cord repair or augmentation, as well as other soft tissue repair or augmentation (e.g., bladder neck augmentation, dermal fillers, breast implants, intervertebral disks, muscle-mass). The hydrogels or compositions thereof are injected into the superficial lamina propria or phonatory epithelium to restore the phonatory mucosa of the vocal cords, thereby restoring a patient's voice. In particular, it has been discovered that hydrogels with an elastic shear modulus of approximately 25 Pa are useful in restoring the pliability of the phonatory mucosa. The invention also provides methods of preparing and using the inventive hydrogels.
摘要翻译： 本发明提供水凝胶及其组合物，用于声带修复或增强，以及其它软组织修复或增强（例如，膀胱颈增大，皮肤填充剂，乳房植入物，椎间盘，肌肉质量）。 将水凝胶或其组合物注射到表层固有层或声音上皮中以恢复声带的声音粘膜，从而恢复患者的声音。 特别地，已经发现弹性剪切模量为约25Pa的水凝胶可用于恢复发音粘膜的柔韧性。 本发明还提供了制备和使用本发明水凝胶的方法。
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公开(公告)号：US08808681B2
公开(公告)日：2014-08-19
申请号：US11758078
申请日：2007-06-05
IPC分类号： A61K47/32 , A61K35/00 , C08F2/48 , C08G73/00 , C08F299/04 , C08F290/06 , C08F283/00 , C08G73/02 , C08F299/02 , C08L79/02 , C08G63/685
CPC分类号： C08L79/02 , C08F283/00 , C08F290/06 , C08F290/061 , C08F290/065 , C08F299/024 , C08F299/0485 , C08G63/6858 , C08G73/02
摘要： Acrylate-terminated poly(beta-amino esters) are cross-linked to form materials useful in the medical as well as non-medical field. The polymeric starting material is combined with a free radical initiator, either a thermal initiator or a photoinitiator, and the mixture for cross-linking is heated or exposed to light depending on the initiator used. The resulting materials due to the hydrolysable ester bond in the polymer backbone are biodegradable under physiological conditions. These cross-linked materials are particular useful as drug delivery vehicles, tissue engineering scaffolds, and in fabricating microdevices. The materials may also be used as plastics, coating, adhesives, inks, etc. The cross-linked materials prepared exhibit a wide range of degradation times, mass loss profiles, and mechanical properties. Therefore, the properties of the material may be tuned for the desired use. The high-throughput approach to preparing a library of cross-linked poly(beta-amino esters) allows for the rapid screening and design of degradable polymers for a variety of applications.
摘要翻译： 丙烯酸酯封端的聚（β-氨基酯）被交联以形成用于医疗和非医学领域的材料。 将聚合起始材料与自由基引发剂，热引发剂或光引发剂组合，并且根据所使用的引发剂将用于交联的混合物加热或暴露于光。 由于聚合物骨架中的可水解的酯键导致的所得材料在生理条件下是可生物降解的。 这些交联材料特别用作药物递送载体，组织工程支架和制造微型装置。 这些材料也可以用作塑料，涂料，粘合剂，油墨等。制备的交联材料表现出广泛的降解时间，质量损失曲线和机械性能。 因此，可以调整材料的性质以达到期望的用途。 制备交联聚（β-氨基酯）文库的高通量方法允许快速筛选和设计可降解聚合物用于各种应用。
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公开(公告)号：US08637028B2
公开(公告)日：2014-01-28
申请号：US13123888
申请日：2009-10-09
申请人： Frank Alexis , Matteo Iannacone , Jinjun Shi , Pamela Basto , Elliott Ashley Moseman , Ulrich von Andrian , Robert S. Langer , Omid C. Farokhzad , Elena Tonti
发明人： Frank Alexis , Matteo Iannacone , Jinjun Shi , Pamela Basto , Elliott Ashley Moseman , Ulrich von Andrian , Robert S. Langer , Omid C. Farokhzad , Elena Tonti
IPC分类号： A61K39/40 , A61K39/395 , A61K49/04
CPC分类号： A61K9/14 , A61K38/20 , A61K38/21 , A61K39/00 , A61K39/0002 , A61K39/002 , A61K39/05 , A61K39/08 , A61K39/12 , A61K39/39 , A61K45/06
摘要： The present invention provides compositions and systems for delivery of nanocarriers to cells of the immune system. The invention provides nanocarriers capable of stimulating an immune response in T cells and/or in B cells. The invention provides nanocarriers that comprise an immunofeature surface and an immunostimulatory moiety. In some embodiments, the immunostimulatory moiety is an adjuvant. The invention provides pharmaceutical compositions comprising inventive nanocarriers. The present invention provides methods of designing, manufacturing, and using inventive nanocarriers and pharmaceutical compositions thereof.
摘要翻译： 本发明提供用于将纳米载体递送至免疫系统细胞的组合物和系统。 本发明提供能够刺激T细胞和/或B细胞中的免疫应答的纳米载体。 本发明提供包含免疫表面和免疫刺激部分的纳米载体。 在一些实施方案中，免疫刺激部分是佐剂。 本发明提供包含本发明纳米载体的药物组合物。 本发明提供了设计，制造和使用本发明的纳米载体及其药物组合物的方法。
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