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公开(公告)号:US5254802A
公开(公告)日:1993-10-19
申请号:US625326
申请日:1990-12-10
摘要: Brassica oleracea plants having cytoplasmic male sterility and processes for their production.
摘要翻译: 芸苔属植物具有细胞质雄性不育及其生产过程。
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公开(公告)号:US07029908B1
公开(公告)日:2006-04-18
申请号:US09469812
申请日:1999-12-22
申请人: Maarten Hendrik Stuiver , Anne Silene Ponstein , Stephan Andreas Ohl , Oscar Johanna Maria Goddijn , Lambertus Henricus Simons , Bernardus Martinus Maria Dekker , Sietske Hoekstra , Hendrik Tigelaar , Nicolas Elzinga
发明人: Maarten Hendrik Stuiver , Anne Silene Ponstein , Stephan Andreas Ohl , Oscar Johanna Maria Goddijn , Lambertus Henricus Simons , Bernardus Martinus Maria Dekker , Sietske Hoekstra , Hendrik Tigelaar , Nicolas Elzinga
IPC分类号: C12N15/82
CPC分类号: C12N15/8205 , C12N15/8201
摘要: The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
摘要翻译: 本发明提供用于农杆菌药物植物细胞转化的新型Ti-质粒载体。 本发明的扁桃体构建在T-DNA边界基因侧翼侧的DNA序列(即,抑制DNA解链的负选择性制备者或DNA序列),其减少或消除了载体读取DNA的转化体的出现 序列。 本发明还提供利用这些新载体的植物细胞转化方法。
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