公开(公告)号：US20240327856A1

公开(公告)日：2024-10-03

申请号：US18618853

申请日：2024-03-27

Applicant: Benson Hill, Inc.

Inventor： Matthew Brett Begemann ,  Herbert Wolfgang Goettel ,  Elizabeth May Frick ,  Erin Kathleen Zess

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/8245 ,  C12N9/22 ,  C12N15/11 ,  C12N15/8201 ,  C12N2310/20

Abstract: Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein and/or oil composition) comprising reduced galactinol synthase (GAS) and/or raffinose synthase (RS) activity, and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the GAS and/or RS activity, decreased RFO (e.g., raffinose, stachyose) content, increased sucrose content, and/or increased protein content. The mutation can be located at least partially in a GAS and/or RS gene or its homolog or its regulatory region.

公开(公告)号：US12018272B2

公开(公告)日：2024-06-25

申请号：US18296579

申请日：2023-04-06

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/63 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  A61K48/00

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11807858B2

公开(公告)日：2023-11-07

申请号：US17118539

申请日：2020-12-10

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10 ,  A01H6/20 ,  A01H6/54 ,  A01H6/46 ,  A01H6/60 ,  C12N1/20 ,  C12R1/41

CPC classification number: C12N15/8209 ,  A01H4/003 ,  A01H4/008 ,  C12N5/0025 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8221 ,  C12N15/8265 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  G01N33/0098 ,  A01H6/202 ,  A01H6/4684 ,  A01H6/542 ,  A01H6/604 ,  C12N1/205 ,  C12N15/8261 ,  C12R2001/41 ,  C12Y203/01081

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20230328020A1

公开(公告)日：2023-10-12

申请号：US18324926

申请日：2023-05-26

Applicant: Salk Institute for Biological Studies

Inventor： Patrick D. Hsu ,  Silvana Konermann

IPC: H04L51/212 ,  C12N15/10 ,  C12N15/113 ,  C12N15/85 ,  C12N15/82 ,  C12N9/22 ,  H04L51/214 ,  H04L9/08 ,  H04L9/30 ,  H04L45/745 ,  H04L51/08 ,  H04L51/18

CPC classification number: H04L51/212 ,  C12N15/102 ,  C12N15/113 ,  C12N15/85 ,  C12N15/8201 ,  C12N9/22 ,  C12N15/8213 ,  H04L51/214 ,  H04L9/08 ,  H04L9/30 ,  H04L45/745 ,  H04L51/08 ,  H04L51/18 ,  C12N2310/3519 ,  C12N2310/20 ,  C12N2310/16

Abstract: Provided herein are CRISPR/Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit, or modify a target RNA.

公开(公告)号：US20230323373A1

公开(公告)日：2023-10-12

申请号：US18025530

申请日：2021-09-07

Applicant: BASF Plant Science Company GmbH

Inventor： Yuki MATSUBA ,  Vinitha CARDOZA ,  Baochun LI ,  Marianela RODRIGUEZ ,  Joerg BAUER ,  Keiji NUMATA ,  Jordan Corinne THERIOT ,  Chonprakun THAGUN

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8201

Abstract: The invention relates to a complex comprising a Cell Penetrating Peptide and one or more nucleic acids which can be applied to a plant by spraying and which can trigger a physiological outcome. Hereto, the one or more nucleic acids complex with a Cell Penetrating Peptide can be dissolved in water without the presence of additional components in the solution.

公开(公告)号：US20230287372A1

公开(公告)日：2023-09-14

申请号：US18006324

申请日：2021-07-20

Applicant: BRAIN Biotech AG

Inventor： Paul SCHOLZ ,  Christian ZUREK ,  Michael KROHN

IPC: C12N9/22 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/8201 ,  C12N2310/20

Abstract: The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90%, preferably at least 92 %, and most preferably at least 95% identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90%, preferably at least 92%, and most preferably at least 95% identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

公开(公告)号：US20230279369A1

公开(公告)日：2023-09-07

申请号：US18164700

申请日：2023-02-06

Applicant: SUMITOMO RUBBER INDUSTRIES, LTD. ,  TOHOKU UNIVERSITY ,  KANAZAWA UNIVERSITY ,  NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY

Inventor： Haruhiko YAMAGUCHI ,  Yukino INOUE ,  Seiji TAKAHASHI ,  Toru NAKAYAMA ,  Satoshi YAMASHITA ,  Yuzuru TOZAWA

IPC: C12N9/10 ,  C12N15/52 ,  C12P5/00 ,  C12N15/82

CPC classification number: C12N9/1085 ,  C12Y205/0102 ,  C12N15/52 ,  C12P5/007 ,  C12N15/8201 ,  C12N2510/00

Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.

公开(公告)号：US20230242926A1

公开(公告)日：2023-08-03

申请号：US17934089

申请日：2022-09-21

Applicant: KWS SAAT SE & Co. KGaA

Inventor： Hinrich HARLING ,  Susana MARTIN-ORTIGOSA ,  Markus NIESSEN ,  Corinna STREITNER ,  Nadine SCHUMANN ,  Erik JONGEDIJK

IPC: C12N15/82 ,  C12N15/113 ,  A01H5/04 ,  A01H5/12

CPC classification number: C12N15/8213 ,  A01H5/04 ,  A01H5/12 ,  C12N15/113 ,  C12N15/8201 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8207 ,  C12N2310/20

Abstract: The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and/or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.

公开(公告)号：US20190249193A1

公开(公告)日：2019-08-15

申请号：US16397213

申请日：2019-04-29

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/82 ,  C12N15/81 ,  C12N9/22 ,  C12N15/90 ,  C12N15/63 ,  C12N15/87 ,  C12N15/01

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20190249187A1

公开(公告)日：2019-08-15

申请号：US16308852

申请日：2017-04-07

Applicant: BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. ,  NANJING AGRICULTURAL UNIVERSITY

Inventor： Jian He ,  Li Yao ,  Xingjun Jia ,  Xiangting Xie ,  Yechun Wu ,  Qing Tao ,  Derong Ding

IPC: C12N15/82 ,  C12N15/62 ,  C07K14/415

CPC classification number: C12N15/8274 ,  A01G13/00 ,  A01H5/00 ,  C07K14/415 ,  C12N5/10 ,  C12N15/62 ,  C12N15/82 ,  C12N15/8201

Abstract: Provided are a resistant protein for use in herbicide dicamba, encoding gene and application thereof, the gene comprising: (a) a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO: 2; or (b) a nucleotide sequence which is complementary to the nucleotide sequence as defined by (a) under stringent conditions; or (c) a nucleotide sequence as shown in SEQ ID NO: 1.