SELECTION METHOD
    2.
    发明申请
    SELECTION METHOD 审中-公开
    选择方法

    公开(公告)号:US20100186125A1

    公开(公告)日:2010-07-22

    申请号:US12448263

    申请日:2007-12-14

    IPC分类号: A01H5/00 C12N15/82 C12N5/10

    CPC分类号: C12N15/8214

    摘要: The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.

    摘要翻译: 本发明涉及一种生产转化植物细胞的方法。 更具体地,该方法涉及用靶向植物质体的转化盒转化植物细胞,其包含选择基因,例如异戊烯基转移酶(IPT)和转基因。 在选择转化质体后,在植物细胞中诱导重组酶的表达,这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。