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公开(公告)号：US06330058B1
公开(公告)日：2001-12-11
申请号：US09548569
申请日：2000-04-13
IPC分类号： G01N3348
摘要： A method and apparatus for characterizing the type of a blood sample are provided wherein an optical density spectrum of the sample is collected over a predetermined wavelength range. A reference optical density spectrum is collected over a predetermined wavelength range for a portion of the blood sample diluted in saline. Another portion of the blood sample is then mixed with an antibody corresponding to a known blood type (e.g., anti-A, anti-B, anti-D antibody). The optical density spectrum is then collected over a predetermined wavelength range for blood diluted with saline and each antibody in saline. The slopes are then calculated over a predetermined wavelength range for each spectrum. A numerical indicator of agglutination is then calculated by dividing the slope of each antibody-treated sample by the slope of the sample in saline. The resulting number is multiplied by 100. The agglutination index (AI) is arrived at by subtracting this number from 100 so that the magnitude of the AI is a reflection of the degree of agglutination of the sample. A high index value indicates large agglutination (i.e., strong interaction with antibody). Blood type is determined by comparing the AI to a predetermined empirical cutoff value. Typically cutoff values greater than 17 indicate type-specific interaction (type AB samples yield AI values over 17 with both anti-A and anti-B antibodies, while type O samples yield AI values less than 17 with both anti-A and anti-B antibodies).
摘要翻译： 提供了用于表征血液样本的类型的方法和装置，其中在预定波长范围内收集样品的光密度谱。 对于在盐水中稀释的部分血液样品，在预定波长范围内收集参考光密度光谱。 然后将另一部分血液样品与对应于已知血型（例如抗A，抗B，抗-D抗体）的抗体混合。 然后在预定波长范围内收集用盐水稀释的血液和盐水中的每种抗体的光密度谱。 然后在每个频谱的预定波长范围内计算斜率。 然后通过将每个抗体处理的样品的斜率除以盐水中样品的斜率来计算凝集数值指标。 结果数乘以100.通过从100减去该数值得到凝集指数（AI），使得AI的大小反映样品的凝集程度。 高指数值表示大的凝集（即与抗体的强相互作用）。 通过将AI与预定经验截断值进行比较来确定血型。 通常，大于17的截止值表示类型特异性相互作用（AB型样品产生具有抗A和抗B抗体的17个以上的AI值，而O型样品在抗A和抗B两者中产生小于17的AI值 抗体）。
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