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公开(公告)号：US09029042B2
公开(公告)日：2015-05-12
申请号：US12678687
申请日：2008-09-24
CPC分类号： H01M8/188 , Y02E60/528
摘要： This invention provides a redox fuel cell comprising an anode and a cathode separated by an ion selective polymer electrolyte membrane; means for supplying a fuel to the anode region of the cell; means for supplying an oxidant to the cathode region of the cell; means for providing an electrical circuit between the anode and the cathode; a non-volatile catholyte solution flowing fluid communication with the cathode, the catholyte solution comprising a polyoxometallate redox couple being at least partially reduced at the cathode in operation of the cell, and at least partially re-generated by reaction with the oxidant after such reduction at the cathode, the catholyte solution comprising at least one counterion for the polyoxometallate redox couple wherein the at least one counterion comprises one or more divalent ions.
摘要翻译： 本发明提供了一种氧化还原燃料电池，其包括由离子选择性聚合物电解质膜分离的阳极和阴极; 用于向所述电池的阳极区域供应燃料的装置; 用于向所述电池的阴极区域供应氧化剂的装置; 用于在阳极和阴极之间提供电路的装置; 与阴极流体连通的非挥发性阴极电解液，包含多金属氧酸盐氧化还原对的阴极电解液在电解槽操作时在阴极处至少部分还原，并且在这种还原之后至少部分地与氧化剂反应产生重新产生 在阴极处，阴极电解液包含至少一种用于多金属氧酸盐氧化还原对的抗衡离子，其中所述至少一种抗衡离子包含一个或多个二价离子。
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公开(公告)号：US08541219B2
公开(公告)日：2013-09-24
申请号：US12765253
申请日：2010-04-22
申请人： Robert Potter , Kim Rosenthal
发明人： Robert Potter , Kim Rosenthal
CPC分类号： C12N9/1276 , C12Q2521/107
摘要： The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.
摘要翻译： 本发明涉及具有增加的保真度（或降低的错配率）和/或末端脱氧核苷酸转移酶活性的逆转录酶。 特别地，本发明涉及通过修饰或突变逆转录酶中的指定位置来制备此类逆转录酶的方法。 本发明还涉及含有编码本发明的逆转录酶的基因的宿主细胞含有此类核酸分子的核酸分子以及使用宿主细胞制备逆转录酶的方法。 本发明的逆转录酶特别适用于核酸合成，测序，扩增和cDNA合成。
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公开(公告)号：US20100297522A1
公开(公告)日：2010-11-25
申请号：US12678687
申请日：2008-09-24
IPC分类号： H01M8/10
CPC分类号： H01M8/188 , Y02E60/528
摘要： This invention provides a redox fuel cell comprising an anode and a cathode separated by an ion selective polymer electrolyte membrane; means for supplying a fuel to the anode region of the cell; means for supplying an oxidant to the cathode region of the cell; means for providing an electrical circuit between the anode and the cathode; a non-volatile catholyte solution flowing fluid communication with the cathode, the catholyte solution comprising a polyoxometallate redox couple being at least partially reduced at the cathode in operation of the cell, and at least partially re-generated by reaction with the oxidant after such reduction at the cathode, the catholyte solution comprising at least one counterion for the polyoxometallate redox couple wherein the at least one counterion comprises one or more divalent ions.
摘要翻译： 本发明提供了一种氧化还原燃料电池，其包括由离子选择性聚合物电解质膜分离的阳极和阴极; 用于向所述电池的阳极区域供应燃料的装置; 用于向所述电池的阴极区域供应氧化剂的装置; 用于在阳极和阴极之间提供电路的装置; 与阴极流体连通的非挥发性阴极电解液，包含多金属氧酸盐氧化还原对的阴极电解液在电解槽操作时在阴极处至少部分还原，并且在这种还原之后至少部分地与氧化剂反应产生重新产生 在阴极处，阴极电解液包含至少一种用于多金属氧酸盐氧化还原对的抗衡离子，其中所述至少一种抗衡离子包含一个或多个二价离子。
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公开(公告)号：US20070020622A1
公开(公告)日：2007-01-25
申请号：US10244081
申请日：2002-09-16
申请人： Jun Lee , Gary Gerard , Harini Shandilya , Katherine Griffiths , Moreland Gibbs , Peter Bergquist , Robert Potter
发明人： Jun Lee , Gary Gerard , Harini Shandilya , Katherine Griffiths , Moreland Gibbs , Peter Bergquist , Robert Potter
CPC分类号： C12N9/1252 , C12N9/1276 , C12N9/14 , C12P19/34 , C12Q1/6834 , C12Q1/6869 , C12Y207/07007 , C12Y306/01 , C12Q2521/101
摘要： The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may posses both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.
摘要翻译： 本发明提供具有核苷酸聚合酶活性的多肽和增强聚合酶活性的方法。 本发明的多肽可以具有DNA依赖性DNA聚合酶活性和RNA依赖性DNA聚合酶活性，即逆转录酶活性。 本发明的多肽可用于任何应用，包括但不限于DNA测序反应，扩增反应，cDNA合成反应以及合并的cDNA合成和扩增反应，例如RT-PCR。
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公开(公告)号：US20060094050A1
公开(公告)日：2006-05-04
申请号：US11265159
申请日：2005-11-03
申请人： Robert Potter , Kim Rosenthal
发明人： Robert Potter , Kim Rosenthal
CPC分类号： C12N9/1276 , C12Q2521/107
摘要： The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.
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6.发明授权Spectrophotometric method for determining the viability of a sample containing platelets 有权用于测定含有血小板的样品的活力的分光光度法公开(公告)号：US06984526B2
公开(公告)日：2006-01-10
申请号：US09861781
申请日：2001-05-21
申请人： Luis Humberto Garcia-Rubio , Robert Potter , German Leparc , Sharyn Orton , Yvette Mattley , Christina Bacon
发明人： Luis Humberto Garcia-Rubio , Robert Potter , German Leparc , Sharyn Orton , Yvette Mattley , Christina Bacon
IPC分类号： G01N21/62
CPC分类号： G01N21/31 , G01N21/49 , G01N33/80 , Y10T436/101666 , Y10T436/106664 , Y10T436/110833 , Y10T436/112499 , Y10T436/115831 , Y10T436/12 , Y10T436/25125
摘要： A method and apparatus for characterizing the type of a blood sample and a variety of blood components are provided wherein a transmission spectrum of the sample is collected over a predetermined wavelength range. For blood typing, this spectrum is then compared with a set of control spectra collected from control blood samples having known blood types, from which the type of the blood sample can be determined. Further methods and apparatus are provided for determining the viability of and for cross matching a platelet unit. Additional method and apparatus permit analysis of the sample for the presence of a contaminant. Particles can also be counted in the sample, even when present in low concentrations, including white blood cell.
摘要翻译： 提供了用于表征血液样本的类型和各种血液成分的方法和装置，其中在预定波长范围上收集样品的透射光谱。 对于血型分析，然后将该光谱与从已知血型对照血液样品收集的一组对照光谱进行比较，从该血液样品中可以确定血液样品的类型。 提供了进一步的方法和装置，用于确定血小板单元的活力和交叉匹配。 附加的方法和装置允许对样品的污染物的存在进行分析。 即使以低浓度存在，包括白细胞，也可以在样品中计数颗粒。
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公开(公告)号：US20060292578A1
公开(公告)日：2006-12-28
申请号：US11170762
申请日：2005-06-28
申请人： Weidong Zheng , Jun Lee , Robert Potter , David Mandelman
发明人： Weidong Zheng , Jun Lee , Robert Potter , David Mandelman
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Y207/07007 , Y02P20/52 , C12Q2561/101 , C12Q2527/149 , C12Q2521/101
摘要： A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,
摘要翻译： 一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。 该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法，
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8.发明申请Enhancement of nucleic acid amplification using double-stranded DNA binding proteins 审中-公开使用双链DNA结合蛋白增强核酸扩增公开(公告)号：US20060275792A1
公开(公告)日：2006-12-07
申请号：US11365383
申请日：2006-02-28
CPC分类号： C12Q1/686 , C12Q1/6848 , C12Q2563/107 , C12Q2535/125 , C12Q2525/15
摘要： Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.
摘要翻译： 公开了用于增强核酸扩增反应的效率，特异性和/或特异性的方法和材料（例如，PCR扩增反应）。 所公开的发明涉及在扩增反应中使用非特异性双链DNA结合蛋白。 本文描述的方法可用于各种分子生物学应用，包括等位基因特异性PCR。
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公开(公告)号：US20060030007A1
公开(公告)日：2006-02-09
申请号：US10857435
申请日：2004-06-01
申请人： Devon Byrd , Dominic Esposito , Robert Potter , Thomas Chappell
发明人： Devon Byrd , Dominic Esposito , Robert Potter , Thomas Chappell
IPC分类号： C07H21/04 , C12P21/04 , C12N9/22 , C07K14/195
CPC分类号： C07K1/22 , C07K2319/00 , C07K2319/20 , C12N9/22
摘要： The invention relates generally to affinity peptides having binding activity for metal ion affinity chromatography media. The invention further relates to vectors which encode these affinity peptides and use of these affinity peptides for the purification of biological molecules such as proteins. The invention also relates to fusion proteins comprising affinity peptides of the invention.
摘要翻译： 本发明一般涉及对金属离子亲和层析介质具有结合活性的亲和肽。 本发明还涉及编码这些亲和肽的载体和这些亲和肽用于纯化诸如蛋白质的生物分子的用途。 本发明还涉及包含本发明的亲和肽的融合蛋白。
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公开(公告)号：US20050130736A1
公开(公告)日：2005-06-16
申请号：US10733648
申请日：2003-12-12
申请人： Willem Polak , Robert Potter
发明人： Willem Polak , Robert Potter
CPC分类号： G07F17/329 , G06Q20/342 , G07F7/025 , G07F17/0014 , G07F17/32 , G07F17/3251
摘要： A prepaid wagering card and a system and method for playing the lottery over a telephone line using the prepaid wagering card are provided. The prepaid wagering card includes information that makes it convenient to obtain the prepaid wagering card and use it to wager sets of numbers listed thereon, while also ensuring that the information remains secure. Participating in a lottery using the prepaid wagering card simply involves calling a wagering system from a telephone and providing requested information. Thus, a user can purchase a prepaid wagering card and decide on which scheduled lottery to actually wager using the prepaid wagering card.
摘要翻译： 提供了一种预付投注卡以及使用预付投注卡通过电话线播放彩票的系统和方法。 预付投注卡包括获取预付投注卡的方便的信息，并使用它来下注其上列出的数字，同时还确保信息保持安全。 使用预付款投注卡参与彩票仅涉及从电话呼叫投注系统并提供所请求的信息。 因此，用户可以购买预付的投注卡，并且使用预付的投注卡来决定实际投注哪个计划的彩票。
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