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公开(公告)号：US4230684A
公开(公告)日：1980-10-28
申请号：US887326
申请日：1978-03-16
申请人： Songja Pang , Maria I. New
发明人： Songja Pang , Maria I. New
CPC分类号： G01N33/76 , Y10S436/811 , Y10S436/817 , Y10S436/825
摘要： A micromethod for the determination of steroids in human body liquids, in particular blood, is disclosed. A small sample of the liquid is transferred to a sheet of absorbing material, e.g., filter paper. A disc of the impregnated paper, containing the dried sample, in eluted with an aqueous solvent, e.g., a buffer solution. Without separating the paper from the elute, the latter is extracted with an organic solvent. A steroid-containing residue is recovered from the organic extract and is subjected to a conventional radioimmunoassay. The method is particularly suited for the detection of disorders with elevated steroid levels such as congenital adrenal hyperplasia in newborn infants by determining the 17.alpha.-hydroxy-progesterone concentration in small blood samples.
摘要翻译： 公开了用于测定人体液体，特别是血液中类固醇的微量方法。 将一小部分液体样品转移到一张吸收材料（例如滤纸）上。 含有干燥样品的浸渍纸盘，用水溶剂例如缓冲溶液洗脱。 不将纸与洗脱液分离，后者用有机溶剂萃取。 从有机提取物中回收含类固醇的残留物，并进行常规的放射免疫测定。 该方法特别适用于通过测定小血液样品中17α-羟基孕酮浓度来检测新生儿中先天性肾上腺增生的类固醇水平升高的疾病。
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公开(公告)号：US4431743A
公开(公告)日：1984-02-14
申请号：US201589
申请日：1980-10-28
申请人： Songja Pang , Maria New
发明人： Songja Pang , Maria New
CPC分类号： G01N33/76 , Y10S436/804 , Y10S436/811 , Y10S436/817
摘要： A method for determination of a steroid such as dehydroepiandrosterone sulfate (DS) in a sample of a human body liquid wherein the liquid sample is transferred to a sheet of microfilter paper and dried before being treated with an aqueous solvent to obtain a mixture wherein the dried body liquid is substantially redissolved in the aqueous solution. The mixture is contacted with an aqueous solution of an agent capable of selectively binding the steroid in the presence of a radioisotopically labeled form of steriod whereby part of the labeled steroid and part of the unlabeled steroid present in the sample are bound by forming a complex with the binding agent. Bound steroids are separated from unbound steroids in the aqueous solution and the radioactivity of at least the separated binding agent-steroids-complex or the unbound steroids is performed to determine the concentration of the hormone as a function of measured radioactivity. Additionally, a means for performing the method is disclosed.
摘要翻译： 一种在人体液体样品中测定类固醇如脱氢表雄酮硫酸盐（DS）的方法，其中将液体样品转移到一片微过滤纸上，并在用水溶剂处理之前干燥以获得其中干燥 体液基本上重新溶解在水溶液中。 将混合物与能够在放射性同位素标记形式的甾体化合物存在下选择性结合类固醇的试剂的水溶液接触，由此通过与样品中形成复合物的方式结合标记的类固醇和样品中存在的部分未标记的类固醇 粘合剂。 结合的类固醇与水溶液中未结合的类固醇分离，至少分离的结合剂 - 类固醇复合物或未结合的类固醇的放射活性进行，以确定激素的浓度作为测量放射性的函数。 此外，公开了一种用于执行该方法的装置。
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