公开(公告)号：US08129145B2

公开(公告)日：2012-03-06

申请号：US11827301

申请日：2007-07-11

Applicant: Daniel R. Lasko ,  Stephan M. Koza

Inventor： Daniel R. Lasko ,  Stephan M. Koza

IPC: C12P21/04 ,  C07K14/00 ,  C12N5/06

CPC classification number: C12N5/0018 ,  C07K16/18 ,  C07K2317/41 ,  C12N5/0037 ,  C12N2500/20 ,  C12N2510/02

Abstract: An improved system for large scale production of glycoproteins in cell culture is provided. In accordance with the present invention, cells expressing a glycoprotein are grown in media that contain manganese at a concentration of between approximately 10 and 600 nM. The use of such a system allows production of a glycoprotein with an increased glycosylation pattern and/or a glycosylation pattern that more accurately reflects the glycosylation pattern of the naturally occurring glycoprotein. A glycoprotein expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical compositions.

Abstract translation: 提供了一种用于细胞培养中大规模生产糖蛋白的改进体系。 根据本发明，表达糖蛋白的细胞在含有约10至600nM浓度的锰的培养基中生长。 使用这种系统允许生产具有更准确地反映天然存在的糖蛋白的糖基化模式的增加的糖基化模式和/或糖基化模式的糖蛋白。 根据本发明表达的糖蛋白可有利地用于制备药物组合物。